A Robust Procedure for Determination of Immunosuppressants Cyclosporine A and Tacrolimus in Blood Samples with Detection of LC–MS/MS

The immunosuppressants monitoring is crucial in the treatment of transplant patients to avoid the risk of organ rejection. So it is vital to develop an accurate method to obtain precise and reliable results. Herein, a liquid chromatography tandem mass spectrometry (LC–MS/MS) based method was develop...

Full description

Saved in:
Bibliographic Details
Published in:Chromatographia 2023-03, Vol.86 (3), p.255-263
Main Authors: Huo, Yumei, Zhang, Shangqing, Pan, Chao, Shan, Hongbo, Xu, Yuxuan, Cai, Xin
Format: Article
Language:English
Subjects:
Analytical Chemistry
Blood
Chemistry
Chemistry and Materials Science
Chromatography
Correlation coefficients
Immunosuppressive agents
Laboratory Medicine
Linearity
Liquid chromatography
Mass spectrometry
Monitoring
Original
Pharmacy
Proteomics
Scientific imaging
Telemedicine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The immunosuppressants monitoring is crucial in the treatment of transplant patients to avoid the risk of organ rejection. So it is vital to develop an accurate method to obtain precise and reliable results. Herein, a liquid chromatography tandem mass spectrometry (LC–MS/MS) based method was developed and validated, which could simultaneously realize the accurate quantification of cyclosporine A and tacrolimus with a total run time of 4.5 min. Unlike the previously described methods, the highlight of this assay is that there is no using zinc sulfate in the protein precipitation of the whole blood sample, which can effectively prolong the lifespan of mass spectrometry. In addition, the linearity, matrix effect, carryover, accuracy, intraday and interday precision of this method were investigated. The linear range of tacrolimus and cyclosporine were 1–40 ng/mL and 50–2000 ng/mL, respectively; The correlation coefficients ( R 2 ) were both greater than 0.995. The intraday and interday precision of cyclosporine and tacrolimus were ≤ 10%. The lower limit of quantitation (LLOQ) of tacrolimus and cyclosporine A were 1 ng/mL and 50 ng/mL, respectively. The main features of this method are good accuracy, sensitivity, specificity, rapidness, robustness and high throughput. It is suitable for therapeutic drug monitoring of cyclosporine A and tacrolimus in routine clinical diagnostics.
ISSN:0009-5893
1612-1112
DOI:10.1007/s10337-023-04241-8