A potato intragene overexpressing GSL1 confers resistance to Pectobacterium atrosepticum

An intragene involves the rearrangement of gene configurations based on switching coding sequences and regulatory regions within a species. The overexpression of specific GSL (Gibberellin Stimulated-Like) genes is known to confer enhanced disease resistance. A potato intragenic expression cassette,...

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Bibliographic Details
Published in:New Zealand journal of crop and horticultural science 2023-04, Vol.51 (2), p.212-230
Main Authors: Jacobs, Jeanne M. E., Meiyalaghan, Sathiyamoorthy, Mohan, Sara, Latimer, Julie M., Thompson, Michelle L., Monaghan, Katrina S., Jones, E. Eirian, Conner, Anthony J.
Format: Article
Language:English
Subjects:
Bioassays
Blackleg
Blackleg disease
Cultivars
Disease resistance
Gene rearrangement
Gene transfer
Gibberellin Stimulated-Like proteins
Gibberellins
Gsl1 gene
High resistance
intragene
Pathogenicity
Pathogens
Pectobacterium
Pectobacterium atrosepticum
Plant bacterial diseases
Potatoes
Regulatory sequences
Snakin 1
Solanum tuberosum
Vegetables
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Summary:An intragene involves the rearrangement of gene configurations based on switching coding sequences and regulatory regions within a species. The overexpression of specific GSL (Gibberellin Stimulated-Like) genes is known to confer enhanced disease resistance. A potato intragenic expression cassette, with the potato GSL1 gene placed under the regulatory control of the light-inducible Lhca3 gene from potato, was designed and constructed. The resulting intragene was transformed using Agrobacterium-mediated gene transfer into potato cultivar Iwa to investigate whether overexpression of the potato GSL1 intragene can confer disease resistance. The transgenic status of the resulting plants was confirmed by PCR. The majority of the transgenic potato lines were determined to overexpress the GSL1 gene at the mRNA level using quantitative RT-PCR analysis. From these results, seven lines were selected for further characterisation and thoroughly evaluated in bioassays for resistance to Pectobacterium atrosepticum which causes blackleg disease in potato. These pathogenicity bioassays demonstrated that high transcriptional overexpression of GSL1 confers high resistance to blackleg disease. This confirms a functional role of the GSL1 gene in plant defence against pathogens and provides an intragenic approach for disease resistance in potatoes.
ISSN:0114-0671
1175-8783
DOI:10.1080/01140671.2021.2021954