Impact of haze events on airborne bacterial consortia–a case study

• Published in: SN applied sciences 2021, Vol.3 (1), p.14, Article 14
• Main Authors: Thilakarathne, S. M. N. K., Ekanayake, A., Madamarandawala, P. S., Weerarathne, W. B. C. P., Thotawatthage, C. A., Magana-Arachchi, D. N.
• Format: Article
• Language: English
• Subjects: Air pollution; Air sampling; Anthropogenic factors; Applied and Technical Physics; Bacteria; Chemistry/Food Science; Coal-fired power plants; Consortia; Daytime; Earth and Environmental Sciences: Pollution and Health Impacts; Earth Sciences; Engineering; Environment; Haze; Human influences; Materials Science; Methods; Microorganisms; Outdoor air quality; Polymerase chain reaction; Research Article; Transboundary pollution
• ISSN: 2523-3963; 2523-3971
• DOI: 10.1007/s42452-020-04022-0

Transboundary haze events received a noticeable attention recently, due to their frequent occurrences. They are mainly, consequences of anthropogenic activities. Sri Lanka experienced a haze event parallel to India in November 2019, the first air pollution event in Sri Lanka linked to a haze event in India. Due to the limited availability of information on haze-related microorganisms, we conducted this study in Kandy, Sri Lanka, aiming to explore the airborne bacterial consortia during a haze event. The natural sedimentation method was used for air sampling. Bacterial identification and the total bacterial load were determined using Sanger sequencing and qPCR. Notably, the total bacterial load was elevated by ~ 40% with the haze and decreased with decreasing haze intensity. The highest bacterial load was reported during the day time of the most intense hazy day (1.89 × 10 6 cells/µl) compared to non-hazy days (lowest; 1.12 × 10 5 cells/µl). Twelve bacterial species were identified and the most abundant phylum was Proteobacteria. The most common species observed during haze was Acinetobacter modestus . The percentage of culturable bacterial species was also high during the haze event (75% during day time of the most intense hazy day compared to 25% on the control). Two human pathogenic bacteria Burkholderia multivorans and Chryseobacterium gleum were found only during the haze event. Therefore, haze events could be hazardous to humans by means of the presence and fluctuating amounts of pathogenic bacteria. Thus, these findings are important in developing policies and guidelines to monitor and minimize the negative impact of haze events.