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First report of citrus black spot disease caused by Phyllosticta citricarpa in Benin

Microscopic observation: (a) conidia surrounded by a thin mucoid layer, and (b) conidia with apical appendage (arrow) Koch's postulates were performed in the laboratory on 30 healthy ripe orange fruits, with two replicates, by injecting 5 μl of a suspension of 106 conidia/ml with a sterile medi...

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Bibliographic Details
Published in:New disease reports 2023-01, Vol.47 (1), p.n/a
Main Authors: Toessi, H. G., Ler‐N'Ogn Dadé Amari, G. E., Sikirou, R., Kone, D.
Format: Article
Language:English
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Summary:Microscopic observation: (a) conidia surrounded by a thin mucoid layer, and (b) conidia with apical appendage (arrow) Koch's postulates were performed in the laboratory on 30 healthy ripe orange fruits, with two replicates, by injecting 5 μl of a suspension of 106 conidia/ml with a sterile medical needle approximately 2 mm deep in the albedo of fruit. Pathogenicity test results of Phyllosticta citricarpa on orange fruits: (a) fruit inoculated by Phyllosticta citricarpa, and (b) control fruit inoculated with distilled water Phyllosticta citricarpa (syn. ACKNOWLEDGEMENTS The authors thank the Partnership for Skills in Applied Sciences, Engineering and Technology (PASET) and Regional Scholarship and Innovation Funds (RSIF) programme, the Université Félix Houphouët-Boigny of Côte d'Ivoire, the Institut National des Recherches Agricoles du Bénin (INRAB), the African Centre of Excellence for Climate Change, Biodiversity and Sustainable Agriculture (CEA-CCBAD/Côte d'Ivoire) and the Laboratoire de Phytopathologie du Centre National de Spécialisation en Fruits et Légumes (CNS-FL) /INERA/Burkina-Faso for financial and technical support.
ISSN:2044-0588
2044-0588
DOI:10.1002/ndr2.12145