Visual detection and highly sensitive quantification of antibiotic meropenem in pharmaceutical and human plasma samples using gold nanoparticles

Meropenem is a broad-spectrum β-lactam antibiotic effective against a wide variety of gram-positive and gram-negative organisms, and has been extensively used in the intensive care unit (ICU) for severe infections caused by susceptible microorganisms that are resistant to other available drugs. A no...

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Bibliographic Details
Published in:Separation science and technology 2023-05, Vol.58 (8), p.1540-1551
Main Authors: Nguyen, Quang Khanh, Duong, Minh Ngoc, Nguyen, Thi Bang, Pham, Thi Ngoc Mai
Format: Article
Language:English
Subjects:
Aggregation
Amides
Antibiotics
Blood plasma
Carbonates
Colour
Detection
Gold
gold nanoparticles
HPLC
human plasma
Intensive care
Lactose
Liquid chromatography
Liquid-liquid extraction
Meropenem
Meropenem determination
Methods
Microorganisms
Nanoparticles
Pharmaceuticals
Sodium
Sodium carbonate
Solvent extraction
Specificity
Sucrose
Surface plasmon resonance
UV-Vis absorption
β-Lactam antibiotics
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Summary:Meropenem is a broad-spectrum β-lactam antibiotic effective against a wide variety of gram-positive and gram-negative organisms, and has been extensively used in the intensive care unit (ICU) for severe infections caused by susceptible microorganisms that are resistant to other available drugs. A novel visual detection and highly sensitive method for meropenem quantification has been developed for the first time, based on the aggregation of citrate-capped gold nano particles (AuNPs) induced by the interaction with meropenem, leading to the change in surface plasmon resonance (SPR) properties of AuNPs. By naked eyes, one can detect meropenem trace by the visual change of gold solution color from red to blue, corresponding to the shift of absorbance maximum in the UV-Vis spectrum from 520 nm to 660 nm. Quantification of meropenem is possible through a linear relationship between the optical absorption ratio A 660 /A 520 and the logarithm of meropenem concentrations. The present method has high recovery (97.6-101.5%), high precision (RSD
ISSN:0149-6395
1520-5754
DOI:10.1080/01496395.2023.2189546