Two cases of cartilaginous metaplasia in the sclera of Japanese White rabbits

Spontaneous cartilaginous metaplasia of the sclera has not been reported in rabbits. Herein, we report two cases of spontaneous cartilaginous metaplasia in the sclera of Japanese White (JW) rabbits. Case 1 was noted in a 14-week-old male Kbs:JW rabbit that received a single ocular instillation of 20...

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Bibliographic Details
Published in:Journal of Toxicologic Pathology 2023, Vol.36(1), pp.45-48
Main Authors: Yamada, Kotaro, Yamagiwa, Yoshinori, Masatsugu, Miki, Haranosono, Yu
Format: Article
Language:English
Subjects:
Abnormalities
Assessments
Cartilage
Cell differentiation
Chondrocytes
clera
eye
Fibers
Immunohistochemistry
Lesions
Light emitting diodes
Males
Metaplasia
Necropsy
Neural crest
rabbit
Rabbits
Vimentin
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Summary:Spontaneous cartilaginous metaplasia of the sclera has not been reported in rabbits. Herein, we report two cases of spontaneous cartilaginous metaplasia in the sclera of Japanese White (JW) rabbits. Case 1 was noted in a 14-week-old male Kbs:JW rabbit that received a single ocular instillation of 20% isoproterenol (IP) a day before necropsy, and showed no abnormalities in clinical signs, ophthalmological assessments, and necropsy. Case 2 was noted in a 38-week-old male Kbs:JW rabbit that was housed under light-emitting diode (LED) lighting for 26 weeks and showed no effects of LED on clinical signs, ophthalmological assessments, and necropsy. Histological sections of the eyes of both animals were prepared and stained with hematoxylin and eosin (H&E) and Alcian blue, and immunohistochemical staining for vimentin was performed. The H&E-stained specimens showed focal hyaline cartilage-like tissues distributed between the scleral fibers at the posterior pole in both cases. The surrounding scleral fibers were compressed and/or partially destroyed by the cartilage-like tissue. The cartilage-like matrix was stained blue by Alcian blue, and immunohistochemistry showed that chondrocyte-like cells were positive for vimentin. Based on these findings, we diagnosed cartilaginous metaplasia in the sclera of Kbs:JW rabbits. The lesion was farther from the IP administration site in Case 1 and was not accompanied by other ophthalmological or histopathological abnormalities in either of the cases. This implies that the lesions occurred spontaneously owing to the abnormal differentiation of neural crest-derived cells.
ISSN:0914-9198
1881-915X
1347-7404
DOI:10.1293/tox.2022-0062