Characterization of the Influence of Bovine Serum Albumin (BSA) upon the Electronic Properties and Surface Topography of a Ti-3Cu Biomedical Alloy by Mott-Schottky Analysis (MSA) and Atomic Force Microscopy (AFM)

• Published in: Analytical letters 2023-07, Vol.56 (10), p.1675-1684
• Main Authors: Siddiqui, Muhammad Ali, Jabeen, Aisha, Chandio, Alidad, Tayyab, Sohail, Yang, Ke
• Format: Article
• Language: English
• Subjects: Aatomic force microscopy (AFM); Atomic force microscopy; Barrier layers; Biomedical materials; bovine serum albumin (BSA); Buffers; Cattle; Chemisorption; Mott-Schottky analysis (MSA); Oxide coatings; protein adsorption; Proteins; Serum albumin; Serum proteins; Surface chemistry; Surface roughness; Ti-3Cu alloy; Titanium base alloys; Titanium dioxide; Topography
• ISSN: 0003-2719; 1532-236X
• DOI: 10.1080/00032719.2022.2140163

The electronic characteristics, surface topography, and surface roughness of a Ti-3Cu alloy were investigated in phosphate buffered saline (PBS) containing bovine serum albumin (BSA) at 37 °C and pH 7.4 ± 0.2 by Mott-Schottky analysis (MSA) and atomic force microscopy (AFM). The n-type oxide layer was produced on the Ti-3Cu alloy surface due to the formation of oxygen-deficient TiO 2-x barrier layer with interstitial copper as As the serum-protein concentration increased from 1 g/L to 6 g/L in the phosphate buffered saline, the donor densities (N D ) of the semiconducting oxide layer decreased. The accumulation of serum protein observed by atomic force microscopy became the driving force in the restructuring of the oxide film via chemisorption and caused a reduction in the donor densities. The change in the magnitude of the nano-roughness suggested chemisorption which is beneficial for the osseointegration of Ti-3Cu implants.