Touchable Electrochemical Hydrogel Sensor for Detection of Reactive Oxygen Species‐induced Cellular Senescence in Articular Chondrocytes

• Published in: Advanced functional materials 2023-04, Vol.33 (17), p.n/a
• Main Authors: Kim, Seul Gi, Song, Jinsoo, Ryplida, Benny, Jo, Hyeong Jun, Jeong, Gun‐Jae, Kang, Innie Y., Patel, Jay Milan, Jin, Eun‐Jung, Jang, Young C., Park, Sung Young
• Format: Article
• Language: English
• Subjects: articular chondrocytes; Cartilage; cellular senescence; conductive hydrogels; Degradation; Electronic properties; Hydrogels; Interleukins; Manganese dioxide; Materials science; Oxygen; ROS sensors; Scavenging; Sensors
• ISSN: 1616-301X; 1616-3028
• DOI: 10.1002/adfm.202213887

In this study, a reactive oxygen species (ROS)‐responsive hydrogel sensor (PD/MnO2 hydrogel) is developed that can efficiently detect senescent cells. Using immature murine articular chondrocytes with serial passages, the sensor can identify small interfering RNA (siRNA) knockdown of peroxisome proliferator‐activated receptor‐alpha (PPARα) based on the concentration of ROS in cells, simultaneously maintaining its balance via scavenging activity to prevent cartilage degradation in osteoarthritis (OA). The hydrogel sensor exhibits a change in electronic properties, with a distinct resistance from 201.9 kΩ for P0 to 362.9 kΩ for P3, and fluorescence off/on performance with an increase in passaging time. In vitro investigation using PPARα‐specific siRNA reveals a correlation between pressure sensitivity and senescent activity, wherein an elevation in observed signal occurred (41.5%). In vivo analysis reveals significant decrease in degradation of the cartilage of both young, 3 months old aged, 18 months old, and PPARα−/− mice compared to PPARα+/+ mice based on safranin O stains. The expression level of interleukin‐1β is reduced in the cartilage of aged PPARα−/− mice after implantation with hydrogel, indicating the potential of PD/MnO2 hydrogel as a therapeutic modality against OA. A hydrogel sensor designed from a polymer dots‐decorated MnO2 nanosheet with controllable electrochemical‐ and fluorescence performance based on the reactive oxygen species (ROS) in tissue/joint environment and the ROS‐responsive conductive hydrogel is developed to wirelessly monitor the senescence process with a significant decrease in degradation of the cartilage.