The Use of Chitosan for Flocculation Recovery of Bacillus Biomass Grown on Dairy and Wine Industry Effluents

The downstream processing of efficient biomass-based microbial biopesticides is heavily reliant on obtaining the largest concentration of viable cells in the most cost-effective manner. The goal of this research was to assess the ability of chitosan flocculation to recover bacterial Bacillus sp. Bio...

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Bibliographic Details
Published in:Processes 2023-04, Vol.11 (4), p.1099
Main Authors: Dmitrović, Selena, Lukić, Nataša, Pajčin, Ivana, Vlajkov, Vanja, Grahovac, Jovana, Jokić, Aleksandar
Format: Article
Language:English
Subjects:
Antibacterial activity
Antimicrobial activity
Antimicrobial agents
Bacillus
Biomass
Biopesticides
Chitosan
Dairy industry
Efficiency
Effluents
Experiments
Factorial design
Flocculation
Flotation
Food
Genetic algorithms
Industrial effluents
Microorganisms
Multiple objective analysis
Optimization
Pathogens
Pesticides
Plant resistance
Response surface methodology
Sedimentation & deposition
Settling velocity
Synergistic effect
Velocity
Wine
Wineries
Wineries & vineyards
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Summary:The downstream processing of efficient biomass-based microbial biopesticides is heavily reliant on obtaining the largest concentration of viable cells in the most cost-effective manner. The goal of this research was to assess the ability of chitosan flocculation to recover bacterial Bacillus sp. BioSol021 biomass from the broth after biological treatment of wastewaters from the dairy and wine industries. Second-order factorial design models were used to estimate the effect of chitosan concentration and mixing speed on flocculation efficiency, settling velocity, and antimicrobial activity against Aspergillus flavus, i.e., inhibition zone diameter. Response surface methodology was followed by multi-objective optimization by applying the desirability function (DF) and genetic algorithm (GA). The optimum values for flocculation efficiency, settling velocity, and inhibition zone diameter for cheese whey effluent were 88%, 0.10 mm/s, and 51.00 mm, respectively. In the case of winery flotation effluent, the optimum values were flocculation efficiency 95% and settling velocity 0.05 mm/s, while the inhibition zone diameter was 48.00 mm. These results indicate that utilizing chitosan as a flocculation agent not only fits the criteria for effective downstream processing, but also has a synergistic effect on Bacillus sp. antibacterial activity.
ISSN:2227-9717
2227-9717
DOI:10.3390/pr11041099