Biohydrogen Production from Enzymatically Digested Cotton Stalks Using Citrobacter freundii

This study presents the production of biohydrogen from acid-treated and enzymatically digested cotton stalks using Citrobacter freundii. Cotton stalks were pre-treated initially using sulphuric acid and hydrolysed using crude cellulase enzyme obtained from Trichoderma reesei . The influence of impor...

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Bibliographic Details
Published in:J. Inst. Eng. (India) ser. E 2023-06, Vol.104 (1), p.11-18
Main Authors: Lakshmidevi, Rajendran, Muthukumar, Karuppan
Format: Article
Language:English
Subjects:
Cellulase
Citrobacter
Cotton
Engineering
Hydrogen production
Original Contribution
Substrates
Sulfuric acid
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Summary:This study presents the production of biohydrogen from acid-treated and enzymatically digested cotton stalks using Citrobacter freundii. Cotton stalks were pre-treated initially using sulphuric acid and hydrolysed using crude cellulase enzyme obtained from Trichoderma reesei . The influence of important parameters such as acid concentration, enzyme dosage, and substrate concentration on the process performance was investigated. A maximum specific hydrogen production rate and hydrogen yield of 126 ml/g of VSS day and 63.8 ml/g cotton waste, respectively, were obtained. The end products formed, and their impact on final pH was also analysed. The morphological changes occurred in cotton stalks after the pre-treatment was analysed using SEM images. The experimental data obtained were fitted with suitable kinetic models to obtain further inferences.
ISSN:2250-2483
2250-2491
DOI:10.1007/s40034-022-00265-1