Effect of dietary Bacillus subtilis supplement on Cd toxicokinetics and Cd-induced immune and antioxidant impairment of Procambarus clarkii

• Published in: Environmental science and pollution research international 2023-03, Vol.30 (15), p.43914-43926
• Main Authors: Dai, Caijiao, Xiao, Lin, Mo, Aijie, Yuan, Yongchao, Yuan, Junfa, Gu, Zemao, Wang, Jianghua
• Format: Article
• Language: English
• Subjects: Acid phosphatase; Alkaline phosphatase; Animals; Antioxidants; Antioxidants - metabolism; Aquatic animals; Aquatic ecosystems; Aquatic Pollution; Astacoidea - chemistry; Atmospheric Protection/Air Quality Control/Air Pollution; Bacillus subtilis; Bacillus subtilis - metabolism; Bioaccumulation; Biodegradation; Biological magnification; Cadmium; Cadmium - toxicity; Catalase; Contaminants; Crayfish; Damage accumulation; Depuration; Dietary Supplements; Earth and Environmental Science; Ecosystem; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; Environmental science; Exoskeleton; Exoskeletons; Exposure; Freshwater ecosystems; Gills; Hemolymph; Hepatopancreas; Lysozyme; Malondialdehyde; Oxidative Stress; Phosphatase; Procambarus clarkii; Research Article; Superoxide dismutase; Toxicokinetics; Waste Water Technology; Water Management; Water Pollution Control
• ISSN: 1614-7499; 0944-1344; 1614-7499
• DOI: 10.1007/s11356-023-25297-x

Cadmium (Cd), a non-biodegradable contaminant in freshwater ecosystems, can pose a serious threat to aquatic animals at high levels. In this study, the Cd toxicokinetics and the immune and antioxidant defense were explored in Procambarus clarkii exposed to different levels of Cd (0, 0.1, 1.0 mg Cd/L) or treated with 1.0 mg Cd/L and dietary Bacillus subtilis supplementation (1 × 10 7  cfu/g). Results from the 21-day uptake and depuration experiment revealed that Cd exposure elicited a dose- and time-dependent uptake in all crayfish tissues, and the rank order of Cd concentration was gill > hepatopancreas > exoskeleton > muscle. The one-compartment model demonstrated that gills had the highest uptake rate ( k u ) value after Cd aqueous exposure and the k u and elimination rate ( k d ) values in gill, hepatopancreas, and exoskeleton of the group with 1.0 mg Cd/L were higher than those of the group at alow Cd concentration (0.1 mg Cd/L). However, B. subtilis could decrease Cd k u and increase Cd k d in hepatopancreas, resulting in the reduction of bioconcentration factors (BCF), steady-state concentrations ( C ss ), and biological half-life ( T b1/2 ). A positive correlation was found between aqueous Cd concentration and the severity of hepatopancreas histopathological injury, while B. subtilis could ameliorate the pathological damage in the high Cd group. Similarly, aqueous exposure to Cd elevated malonaldehyde (MDA) content and suppressed the activities of lysozyme (LZM), acid phosphatase (ACP) in hepatopancreas and alkaline phosphatase (AKP) in hemolymph. The activities of superoxide dismutase (SOD) and catalase (CAT) in hepatopancreas were also inhibited. Nevertheless, they were all recovered with the dietary addition of B. subtilis . In conclusion, our results indicated that exposure to Cd significantly increased Cd accumulation and toxic damages in crayfish hepatopancreas, while dietary administration of B. subtilis to crayfish significantly decreased Cd accumulation and improved the immune and antioxidant defense, leading to the prevention in toxic effects of Cd.