Deep ultraviolet high-resolution microscopic hyperspectral imager and its biological tissue detection

Ultraviolet (UV) hyperspectral imaging technology is commonly used in the field of atmospheric remote sensing. In recent years, some in-laboratory research has been carried out for substance detection and identification. In this paper, UV hyperspectral imaging technology is introduced into microscop...

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Bibliographic Details
Published in:Applied optics (2004) 2023-05, Vol.62 (13), p.3310
Main Authors: Yang, Jingyao, Xue, Qingsheng, Li, Jinze, Han, Bing, Wang, Youpeng, Bai, Haoxuan
Format: Article
Language:English
Subjects:
Animals
Hyperspectral Imaging
Image resolution
Medical imaging
Mice
Microscopy
Nucleic acids
Numerical aperture
Remote sensing
Spatial resolution
Spectral resolution
Tissues
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Summary:Ultraviolet (UV) hyperspectral imaging technology is commonly used in the field of atmospheric remote sensing. In recent years, some in-laboratory research has been carried out for substance detection and identification. In this paper, UV hyperspectral imaging technology is introduced into microscopy to better utilize the obvious absorption characteristics of components, such as proteins and nucleic acids in biological tissues in the ultraviolet band. A deep UV microscopic hyperspectral imager based on the Offner structure with 2.5, low spectral keystone and smile is designed and developed. A 0.68 numerical aperture microscope objective is designed. The spectral range of the system is from 200 nm to 430 nm; the spectral resolution is better than 0.5 nm; and the spatial resolution is better than 1.3 µm. The K562 cells can be distinguished by transmission spectrum of nucleus. The UV microscopic hyperspectral image of the unstained mouse liver slices showed similar results to the microscopic image after hematoxylin and eosin staining, which could help to simplify the pathological examination process. Both results show a great performance in spatial and spectral detecting capabilities of our instrument, which has the potential for biomedical research and diagnosis.
ISSN:1559-128X
2155-3165
DOI:10.1364/AO.485387