Chromatographic Assessment of the Concentration of Phenolic Compounds in Wild Chamomile (Matricaria chamomilla L.) Extracts Obtained under Various Extraction Conditions

Results of a study of water and water–alcohol extracts of wild chamomile ( Matricaria chamomilla L.) depending on the conditions of their extraction by different methods are discussed. The test samples are wild chamomile samples collected in various regions of Russia. Phenolic components in their na...

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Bibliographic Details
Published in:Journal of analytical chemistry (New York, N.Y.) N.Y.), 2023-04, Vol.78 (4), p.497-506
Main Authors: Temerdashev, Z. A., Chubukina, T. K., Vinitskaya, E. A., Kiseleva, N. V.
Format: Article
Language:English
Subjects:
Acetonitrile
Acids
Analysis
Analytical Chemistry
Chamomile
Chemistry
Chemistry and Materials Science
Chlorogenic acid
Chromatography
Composition
Extraction (Chemistry)
Ferulic acid
Formic acid
Glucosides
High performance liquid chromatography
Mass spectrometry
Materia medica, Vegetable
Measurement
Methods
Phenols
Plant extracts
Properties
Raw materials
Spectrometry
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Summary:Results of a study of water and water–alcohol extracts of wild chamomile ( Matricaria chamomilla L.) depending on the conditions of their extraction by different methods are discussed. The test samples are wild chamomile samples collected in various regions of Russia. Phenolic components in their native forms were extracted from medicinal raw materials using infusion, maceration, microwave-assisted, ultrasound-assisted, and subcritical extraction, and by the pharmacopoeial method. Concentrations of individual components and total phenolic compounds in wild chamomile were determined by chromatographic methods. The HPLC determination of phenolic compounds with diode-array and mass spectrometric detection was carried out using gradient elution with a mixture of acetonitrile with 0.1% formic acid. Chlorogenic acid, ferulic acid glycosides, luteolin-7- O -glucoside, dicaffeoylquinic and chicoric acid, apigenin-7-glucoside, and apigenin were identified in water and water–alcohol extracts of wild chamomile. The dependence of the concentration of phenolic compounds on the geographical place of the growth of plant raw materials was studied based on value of this parameter in extracts of wild chamomile samples from different territories. The maximum total concentration of phenolic compounds (10.1 mg/g) was found in the extract of chamomile from FarmaTsvet (Krasnogorsk, Moscow oblast).
ISSN:1061-9348
1608-3199
DOI:10.1134/S1061934823040147