Synthesis of a multi-recognition site borate affinity magnetic surface imprinted polymers for the specific recognition and detection of two glycoproteins at physiological pH

• Published in: Journal of materials science 2023-05, Vol.58 (19), p.7880-7894
• Main Authors: Zhao, Gege, Hu, Cunming, Guan, Ming, Mi, Fang, Wang, Ying
• Format: Article
• Language: English
• Subjects: Adsorption; Affinity; Albumin; Biomarkers; Book publishing; Borates; Boric acid; Characterization and Evaluation of Materials; Chemical Routes to Materials; Chemistry and Materials Science; Classical Mechanics; Copolymers; Crystallography and Scattering Methods; Dopamine; Glycoproteins; Imprinted polymers; Iron oxides; Mass transfer; Materials Science; Medical screening; Nanoparticles; Ovalbumin; Peroxidase; Physiology; Polymer industry; Polymer Sciences; Polymerization; Raman spectra; Recognition; Sandwich structures; Silicon dioxide; Solid Mechanics
• ISSN: 0022-2461; 1573-4803
• DOI: 10.1007/s10853-023-08515-2

Glycoproteins are commonly used as biomarkers for diseases, and capturing multiple target glycoprotein biomarkers at the same time has better specificity than single marker detection in early screening for many diseases. In this work, we propose a method for preparing dual-template magnetic molecularly imprinted nanoparticles (D-MMIPs) with two recognition sites. Firstly, horseradish peroxidase (HRP) and ovalbumin (OVA), two commonly used glycoproteins, were used as double templates to simulate glycoprotein biomarkers. 4-Formylphenyl boric acid (FPBA) was introduced as a functional monomer on the surface of amino-modified Fe 3 O 4 @SiO 2 to fix simultaneously the two templates. Finally, the thin imprinted layer was prepared by surface-controlled polymerization of copolymer of dopamine (DA) and 3-aminophenylboronic acid (APBA), which reduced the mass transfer resistance of the template and improved the eluting efficiency of the template. The obtained D-MMIPs showed a high adsorption capacity of 65.52 mg/g for HRP and 93.61 mg/g for OVA. There is also a satisfactory selectivity in identifying two templates in the real sample under physiological conditions. In addition, a boronate affinity sandwich strategy was constructed by using D-MMIPs as a recognition element combined with surface-enhanced Raman scattering (SERS) technology to achieve rapid separation and detection of HRP and OVA, with detection limits of 2.15 pg/mL and 0.708 pg/mL, respectively. The results imply that the strategy may be suitable for simultaneous and selective capture of multiple glycoprotein biomarkers to detect multiple diseases, and the prepared magnetic imprinting materials have potential value in sensor applications.