Synthesis of Biologically Active Peptides using Newly Designed N-Vinyl Pyrrolidone Incorporated Flexible Cross linked Polystyrene

Synthesis of NS1 and NS2 peptide fragment of hepatitis C virus polyprotein was carried out on a terpolymer- polystyrene, N-vinylpyrrolidone, 1,6-hexanediol diacrylate resin (PS-NVP-HDDA). The radical aqueous suspension polymerisation resulted in the resin, that exhibited high swelling capacity in va...

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Bibliographic Details
Published in:Oriental journal of chemistry 2022-10, Vol.38 (5), p.1217-1226
Main Authors: Jacob, Sunil, Harry, Nissy Ann, K. K. Binoj, K. K. Binoj, Thomas, Preethy Soosan, Mathew, Janey Mary
Format: Article
Language:English
Subjects:
Amino acids
Chemical synthesis
Ethanol
Genomes
Hepatitis C
Homogeneity
Peptides
Polymers
Polystyrene resins
Proteins
Reagents
Resins
Solvents
Suspension polymerization
Swelling
Terpolymers
Vacuum distillation
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Summary:Synthesis of NS1 and NS2 peptide fragment of hepatitis C virus polyprotein was carried out on a terpolymer- polystyrene, N-vinylpyrrolidone, 1,6-hexanediol diacrylate resin (PS-NVP-HDDA). The radical aqueous suspension polymerisation resulted in the resin, that exhibited high swelling capacity in various solvents. The peptides were synthesized step-wise by Fmoc strategy, established with amino acid analysis and purified using HPLC. The polymer's high degree of swelling might facilitate free reagent interaction with resin-bound functional sites, resulting in an increased rate of amide bond production. The peptides yield and purity obtained from new support was high when compared to Merrifield resin. These peptides synthesis depicts the application of PS-NVP-HDDA resin developed for synthesis of long chain peptides in high homogeneity and high yield.
ISSN:0970-020X
2231-5039
DOI:10.13005/ojc/380517