Isolation and identification of flavonoida compound from hujan emas leaves (Galphimia speciosa C. E. Anderson)

• Main Authors: Sembiring, Helmina Br, Zahara, Annisa
• Format: Conference Proceeding
• Language: English
• Subjects: Aromatic compounds; Chemical equilibrium; Chloroform; Chromatography; Column chromatography; Ethyl acetate; Flavonoids; Hexanes; Ketones; Methanol; NMR; Nuclear magnetic resonance; Protons; Thin layer chromatography; Wavelengths
• ISSN: 0094-243X; 1551-7616
• DOI: 10.1063/5.0149806

The isolation and identification of flavonoid had been done from hujan emas (Galphimia speciosa C. E. Anderson) leaves. The dried powder leaves of hujan emas were macerated with methanol and methanol concentrated extract was extracted with ethyl acetate. Ethyl acetate extract was dissolved with methanol and partitioned with n-hexane. The residue was then analyzed by thin layer chromatography and separated by column chromatography with chloroform-methanol (90:10; 80:20; 70:30 and 60: 40) v / v respectively. Compound obtained from fraction 131-145 were purified by preparative thin layer chromatography with chloroform-ethyl acetate (60:40) v / v and obtained 4.8 mg of yellow amorphous solid at an Rf value of 0.2. The UV-Vis spectrum shows a wavelength (λmax ) of 274 nm in band II and 369 nm in band I. The FT-IR spectrum showed the presence of OH, C=C aromatic, C=O ketone, CH sp3.and CO groubs, respectively at wavenumber 3446.79; 2956.87; 1612.89; 1521.84; 1442.75; 1255.66; 829.39 cm−1. The Proton Nuclear Magnetic Resonance (1H-NMR) spectrum show the presence of an H-2, H-2’; H-6’; H-5’; H-8 and H-6 protons which show a chemical shift in the region of 7.73, respectively; 7.65;7.62; 6.89; 6.38; 6.18 ppm on the aromatic ring and methoxy protons which showed a chemical shift in the area of 3.81 ppm. Based on the data obtained, the isolated compound was flavonoid of the isoflavone class.