Exosome loaded hydroxyapatite (HA) scaffold promotes bone regeneration in calvarial defect: an in vivo study

In this study, hydroxyapatite (HA) scaffolds were synthesized and characterized, following the osteogenic and angiogenic effects of HA scaffolds with or without endometrial mesenchymal stem stromal cells (hEnSCs) derived Exosomes were investigated in rat animal model with calvaria defect. The X-ray...

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Bibliographic Details
Published in:Cell and tissue banking 2023-06, Vol.24 (2), p.389-400
Main Authors: Youseflee, Pouya, Ranjbar, Faezeh Esmaeili, Bahraminasab, Marjan, Ghanbari, Ali, Faradonbeh, Davood Rabiei, Arab, Samaneh, Alizadeh, Akram, Nooshabadi, Vajihe Taghdiri
Format: Article
Language:English
Subjects:
Angiogenesis
Animal models
Animals
Biomedical and Life Sciences
Biomedicine
Bone growth
Bone implants
Bone Regeneration
Calvaria
Cell Biology
Cells, Cultured
Durapatite - chemistry
Durapatite - pharmacology
Endometrium
Exosomes
Full Length Paper
Hydroxyapatite
Immunohistochemistry
Life Sciences
Mesenchymal stem cells
Rats
Regeneration
Stromal cells
Tissue engineering
Tissue Scaffolds - chemistry
Transplant Surgery
X-ray diffraction
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Summary:In this study, hydroxyapatite (HA) scaffolds were synthesized and characterized, following the osteogenic and angiogenic effects of HA scaffolds with or without endometrial mesenchymal stem stromal cells (hEnSCs) derived Exosomes were investigated in rat animal model with calvaria defect. The X-ray diffraction (XRD) analysis of HA powder formation was confirmed with Joint Corporation of Powder Diffraction Standards (JCPDS) files numbers of 34-0010 and 24-0033A and Ball mill, and sintering manufactured Nano-size particles. Obtained results containing FE-SEM images presented that the surface of scaffolds has a rough and porous structure, which makes them ideal and appropriate for tissue engineering. Additionally, the XRD showed that these scaffolds exhibited a crystallized structure without undergoing phase transformation; meanwhile, manufactured scaffolds consistently release exosomes; moreover, in vivo findings containing hematoxylin–eosin staining, immunohistochemistry, Masson's trichrome staining, and histomorphometric analysis confirmed that our implant has an osteogenic and angiogenic characteristic. So prepared scaffolds containing exosomes can be proposed as a promising substitute in tissue engineering.
ISSN:1389-9333
1573-6814
DOI:10.1007/s10561-022-10042-4