Characterization of Metabolites in an Endophytic Fungus Diaporthe fraxini via NMR-based Metabolomics and Cholinesterase Inhibitory Activity

Endophytic Diaporthe is a fungal genus having an extensive distribution in plant hosts. It is known as a valuable source of bioactive metabolites with potent biological properties. In the present study, proton nuclear magnetic resonance ( 1 H-NMR) coupled with multivariate analysis (MVA) was employe...

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Bibliographic Details
Published in:Applied biochemistry and microbiology 2023-06, Vol.59 (3), p.316-322
Main Authors: Nagarajan, K., Ibrahim, B., Bawadikji, A.A., Khaw, K.-Y., Tong, W.-Y., Leong, C.-R., Ramanathan, S., Tan, W.-N.
Format: Article
Language:English
Subjects:
Acetylcholinesterase
Bioactive compounds
Biochemistry
Biological properties
Biomedical and Life Sciences
Cholinesterase
Cholinesterase inhibitors
Diaporthe
Endophytes
Fungi
Growth conditions
Host plants
Life Sciences
Magnetic properties
Medical Microbiology
Metabolites
Metabolomics
Microbiology
Multivariate analysis
NMR
Nuclear magnetic resonance
Principal components analysis
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Summary:Endophytic Diaporthe is a fungal genus having an extensive distribution in plant hosts. It is known as a valuable source of bioactive metabolites with potent biological properties. In the present study, proton nuclear magnetic resonance ( 1 H-NMR) coupled with multivariate analysis (MVA) was employed to discriminate the chemical profile of Diaporthe fraxini cultured under different growth conditions. Cholinesterase inhibitory assay was performed to assess the activity of the fungal extracts against acetylcholinesterase and butyrylcholinesterase. Discriminant metabolites responsible for the chemical variations were successfully obtained using 1 H-NMR-based metabolomics approach. Principal component analysis showed a clear discrimination of the fungal extracts of D. fraxini grown under different conditions. Cholinesterase inhibitory activity studies revealed the potential of supplemented cultured fungal extract of D. fraxini as a source of cholinesterase inhibitor.
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683823030134