Extraction optimization using response surface methodology and evaluation of the antioxidant and antimicrobial potential of polyphenols in Scenedesmus sp. and Chlorella sp

• Published in: Biomass conversion and biorefinery 2023-06, Vol.13 (8), p.7185-7198
• Main Authors: Dahmen-Ben Moussa, Ines, Masmoudi, Mohamed Ali, Choura, Sirine, Chamkha, Mohamed, Sayadi, Sami
• Format: Article
• Language: English
• Subjects: Algae; Antiinfectives and antibacterials; Antioxidants; Biotechnology; Chlorella; Energy; Ethanol; Ethyl acetate; Methanol; Optimization; Original Article; Phenols; Polyphenols; Renewable and Green Energy; Response surface methodology; Wastewater
• ISSN: 2190-6815; 2190-6823
• DOI: 10.1007/s13399-021-01850-x

Microalgae are considered natural alternatives to replace synthetic antioxidants. The extraction of polyphenols from Scenedesmus sp. and Chlorella sp. was optimized by employing a central composite design. The effect of solvent nature (ethanol, methanol, and ethyl acetate), extraction temperature (4–80 °C), and extraction time (0.1–4 h) were investigated with an ultrasound-assisted system. Total phenolic compound (TPC) extraction was higher at the combination of methanol, 4 °C, and 4 h for Scenedesmus and methanol, 80 °C, and 4 h for Chlorella . Microalgae were cultivated in BG11 and BG11 + 40% olive mill wastewater (OMUF) media. The extracts were analyzed for their total polyphenol content and antioxidant and antimicrobial activities. The estimation results of models and experimental results indicated a high consistency when cultivating microalgae in BG11 medium. Polyphenols extracted from microalgae cultured with 40% (OMUF) possess significantly high antioxidant and antimicrobial activities. Hence, Scenedesmus sp. and Chlorella sp. showed a capability to grow in the presence of ultrafiltered OMW and produce high added-value compounds.