Alternatives to Pyrethroid Resistance: Combinations of Cymbopogon nardus and Ocimum americanum Essential Oils Improve the Bioefficiency Control Against the Adults' Populations of Aedes aegypti (Diptera: Culicidae)

• Published in: Journal of medical entomology 2022-11, Vol.59 (6), p.2102-2109
• Main Authors: Balboné, Mahamoudou, Soma, Dieudonné Diloma, Drabo, Samuel Fogné, Namountougou, Moussa, Konaté, Hamadou, Meda, Georges Benson, Sawadogo, Ignace, Romba, Rahim, Bilgo, Etienne, Nebié, Roger C. H., Bassolé, Imaël H. N., Dabire, Roch K., Gnankine, Olivier
• Format: Article
• Language: English
• Subjects: Acetic acid; Adults; Aedes; Aedes aegypti; Animals; Aquatic insects; Caryophyllene; Citrates; combination; Cymbopogon - chemistry; Cymbopogon nardus; essential oil; Essential oils; Eucalyptus; Health aspects; Insecticide resistance; Insecticides; Insecticides - chemistry; Insecticides - pharmacology; Larva; major compound; Mortality; Mosquito Vectors; Mosquitoes; Ocimum; Ocimum americanum; Oils & fats; Oils, Volatile - chemistry; Oils, Volatile - pharmacology; p-Cymene; Permethrin; Plant Oils - pharmacology; Plant species; Poaceae; Population studies; Populations; Pyrethrins - pharmacology; Pyrethroids; rate of mortality; resistance; Synergistic effect; Thymol; Toxicity; Tubes; VECTOR CONTROL, PEST MANAGEMENT, RESISTANCE, REPELLENTS; Vector-borne diseases
• ISSN: 0022-2585; 1938-2928
• DOI: 10.1093/jme/tjac148

Dengue vector control strategies are mostly based on chemicals use against Aedes aegypti populations. The current study aimed at investigating the insecticidal effects of essential oils (EOs) obtained from five plant species, Cymbopogon citrates (D. C.) Stapf. (Poaceae), Cymbopogon nardus (Linn.) Rendle (Poaceae), Eucalyptus camaldulensis Linn. (Myrtaceae), Lippia multiflora Moldenke (Verbenaceae), and Ocimum americanum Linn. Lamiaceae, and combinations of Cymbopogon nardus and Ocimum americanum on Ae. aegypti populations from Bobo-Dioulasso. For this purpose, adults of the susceptible and field strains of Ae. aegypti were tested in WHO tubes with EO alone and binary combinations of O. americanum (OA) and C. nardus (CN; scored from C1 to C9). The extraction of the essential oils was done by hydrodistillation, and their components were determined by GC/MS. Among the 5 EOs tested, L. multiflora essential oil was the most efficient, with KDT50 values below 60 min on all Ae. aegypti strains tested, and also with a rate of mortality up to 100 and 85% for Bora Bora and Bobo-Dioulasso strains, respectively. This efficacy may be due to its major compounds which are with major compounds as β-caryophyllene, p-cymene, thymol acetate, and 1.8 cineol. Interestingly, on all strains, C8 combination showed a synergistic effect, while C2 showed an additive effect. These combinations exhibit a rate of mortality varying from 80 to 100%. Their toxicity would be due to the major compounds and the putative combined effects of some major and minor compounds. More importanly, L. multiflora EO and combinations of C. nardus and O. americanum EO, may be used as alternatives against pyrethroid resistant of Ae. aegypti.