Development of rice pre-breeding resources with antioxidant grain properties basing on hybridization and experimental haploidy methods

• Main Authors: Savenko, E. G., Mukhina, Zh. M., Chukhir, I. N., Glazyrina, V. A., Shundrina, L. A.
• Format: Conference Proceeding
• Language: English
• Subjects: Antioxidants; Hybridization; Regeneration; Rice
• ISSN: 0094-243X; 1551-7616
• DOI: 10.1063/5.0140469

12 genotypes from the USU Collection of Federal Scientific Rice Centre were introduced into anther culture – they are donorsof the traits of increased antioxidant activity and recipients (elite varieties of Russian breeding) which were used for hybridization, as well as anthers of seven F1 hybrids obtained from hybridization of samples with colored pericarp and white-grain rice varieties. Morphogenesis on nutritional media was studied in all the samples. 5 genotypes responsive to anther culture technology were selected (VNIIR–10163-6,22/2,89 %; Alliance-2,92/5,45 %; Red-Вlastonik-4,65/1,18%; Veles-3,50/1,03%; К.2327 DH -0,88/1,70%; Heibar DH – 1,01/0,47%, callusogenesis/regeneration respectively). These genotypes formed morphogenic callus with high regeneration ability. In samples with low responsiveness by the trait of “callusogenesis” the intensity of induction processes increased due to the use of subculturing, with the formation of morphogenic callus in the 0th passage at elevated concentrations of the phytohormone 2,4-D. When such callus tissues were transferred to media with a reduced concentration of the phytohormone 2,4-D, the ability to form embryonic complexes occured in the 1st passage. Genotypic variability was noted for the traits of “callugenesis/regeneration” among donors of antioxidant activity and recipient varieties. The rice samples introduced into the crossing had a high combination ability by the trait of “callugenesis”, which was confirmed by the responsiveness to the induction of new growths of the first generation hybrids (F1) in the anther culture in vitro.