Determination of minimal inhibitory concentration of cadmium for Brevibacillus agri C15 and Brevibacillus agri C15 Cdr

Determining resistance by minimal inhibitory concentration (MIC) assay is important in handling bacteria. Here we describe a protocol for identifying the MIC for cadmium (Cd) of Brevibacillus agri C15 and Brevibacillus agri C15 CdR to assist researchers incorrectly confirming the resistance of isola...

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Bibliographic Details
Main Authors: Jebril, Nadia, Boden, Rich, Braungardt, Charlotte
Format: Conference Proceeding
Language:English
Subjects:
Bacteria
Cadmium
Electron microscopy
Granular materials
Morphology
Surface structure
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Summary:Determining resistance by minimal inhibitory concentration (MIC) assay is important in handling bacteria. Here we describe a protocol for identifying the MIC for cadmium (Cd) of Brevibacillus agri C15 and Brevibacillus agri C15 CdR to assist researchers incorrectly confirming the resistance of isolates. In addition, the effects of Cd on the cell-surface structure and morphology, endospore formation, poly-β-hydroxybutyrate and polyphosphate granules were investigated. Its MIC to Cd were 16 ± 0.7 mM and 21± 0.4 mM Cd, respectively. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed no morphological effects of Cd on either of the strains. Both strains have terminal endospores, poly-β-hydroxybutyrate granules and an absence of polyphosphate granules. This projected assay to determine the MIC of cadmium can be applied to the study of new agents with B. agri C15 and B. agri C15 CdR and in laboratory routines for other bacteria.
ISSN:0094-243X
1551-7616
DOI:10.1063/5.0150818