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Protease production by soil bacteria for green technology: Screening and optimization
Protease is a type of enzyme that hydrolyzes proteins into peptides and amino acids. The demand for protease for green technology in some industries like detergent, food, and leather is increasing nowadays. Some soil bacteria, especially the Bacillus strains, are known to have the ability to produce...
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| Published in: | IOP conference series. Earth and environmental science 2023-06, Vol.1201 (1), p.12094 |
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| container_title | IOP conference series. Earth and environmental science |
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| creator | Ratnaningrum, D Kosasih, W Endah, E S Lathifa, A K N Diwan, A M Nida, V Saraswaty, V Risdian, C |
| description | Protease is a type of enzyme that hydrolyzes proteins into peptides and amino acids. The demand for protease for green technology in some industries like detergent, food, and leather is increasing nowadays. Some soil bacteria, especially the
Bacillus
strains, are known to have the ability to produce protease. Compared to other sources like plant and animal origin, microbial protease has more advantages as it can be produced at an industrial scale, short cultivation time, and are easy to harvest. This research aimed to screen the proteolytic bacteria from the bulk soil samples collected under mango trees and to study the optimum condition for protease production using the response surface methodology (RSM). Three bacterial strains (SH2CR, SH3CR, and SC4CR) were isolated and shown to have proteolytic activity. Based on 16S rRNA gene analysis, the strain SH2CR was close to
Priestia megaterium
NBRC 15308
T
(96.97% similarity), while the strains SH3CR and SC4CR were related to
Bacillus zanthoxyli
strain 1433
T
(100% similarity). One of them, SH2CR, was further studied using a fermenter at a one-liter production scale with the medium containing skim milk at 30°C. The best condition for protease production by SH2CR was achieved at 48 h incubation time, 300 rpm of agitation, and 1.25% skim milk. |
| doi_str_mv | 10.1088/1755-1315/1201/1/012094 |
| format | article |
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Bacillus
strains, are known to have the ability to produce protease. Compared to other sources like plant and animal origin, microbial protease has more advantages as it can be produced at an industrial scale, short cultivation time, and are easy to harvest. This research aimed to screen the proteolytic bacteria from the bulk soil samples collected under mango trees and to study the optimum condition for protease production using the response surface methodology (RSM). Three bacterial strains (SH2CR, SH3CR, and SC4CR) were isolated and shown to have proteolytic activity. Based on 16S rRNA gene analysis, the strain SH2CR was close to
Priestia megaterium
NBRC 15308
T
(96.97% similarity), while the strains SH3CR and SC4CR were related to
Bacillus zanthoxyli
strain 1433
T
(100% similarity). One of them, SH2CR, was further studied using a fermenter at a one-liter production scale with the medium containing skim milk at 30°C. The best condition for protease production by SH2CR was achieved at 48 h incubation time, 300 rpm of agitation, and 1.25% skim milk.</description><identifier>ISSN: 1755-1307</identifier><identifier>EISSN: 1755-1315</identifier><identifier>DOI: 10.1088/1755-1315/1201/1/012094</identifier><language>eng</language><publisher>Bristol: IOP Publishing</publisher><subject>Amino acids ; Bacillus ; Bacteria ; Clean technology ; Food industry ; green technology ; Leather ; Microorganisms ; Optimization ; Peptides ; Protease ; Proteinase ; Proteolysis ; Proteolytic bacteria ; Response surface methodology ; rRNA 16S ; Similarity ; Skim milk ; Soil bacteria ; Soil microorganisms ; Strain analysis ; Strains (organisms)</subject><ispartof>IOP conference series. Earth and environmental science, 2023-06, Vol.1201 (1), p.12094</ispartof><rights>Published under licence by IOP Publishing Ltd</rights><rights>Published under licence by IOP Publishing Ltd. This work is published under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3274-738df18312f4fa482ba0240bf651f57b4626463e02c91857103cc22def6cda683</citedby><cites>FETCH-LOGICAL-c3274-738df18312f4fa482ba0240bf651f57b4626463e02c91857103cc22def6cda683</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/2831819637?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,25731,27901,27902,36989,44566</link.rule.ids></links><search><creatorcontrib>Ratnaningrum, D</creatorcontrib><creatorcontrib>Kosasih, W</creatorcontrib><creatorcontrib>Endah, E S</creatorcontrib><creatorcontrib>Lathifa, A K N</creatorcontrib><creatorcontrib>Diwan, A M</creatorcontrib><creatorcontrib>Nida, V</creatorcontrib><creatorcontrib>Saraswaty, V</creatorcontrib><creatorcontrib>Risdian, C</creatorcontrib><title>Protease production by soil bacteria for green technology: Screening and optimization</title><title>IOP conference series. Earth and environmental science</title><addtitle>IOP Conf. Ser.: Earth Environ. Sci</addtitle><description>Protease is a type of enzyme that hydrolyzes proteins into peptides and amino acids. The demand for protease for green technology in some industries like detergent, food, and leather is increasing nowadays. Some soil bacteria, especially the
Bacillus
strains, are known to have the ability to produce protease. Compared to other sources like plant and animal origin, microbial protease has more advantages as it can be produced at an industrial scale, short cultivation time, and are easy to harvest. This research aimed to screen the proteolytic bacteria from the bulk soil samples collected under mango trees and to study the optimum condition for protease production using the response surface methodology (RSM). Three bacterial strains (SH2CR, SH3CR, and SC4CR) were isolated and shown to have proteolytic activity. Based on 16S rRNA gene analysis, the strain SH2CR was close to
Priestia megaterium
NBRC 15308
T
(96.97% similarity), while the strains SH3CR and SC4CR were related to
Bacillus zanthoxyli
strain 1433
T
(100% similarity). One of them, SH2CR, was further studied using a fermenter at a one-liter production scale with the medium containing skim milk at 30°C. The best condition for protease production by SH2CR was achieved at 48 h incubation time, 300 rpm of agitation, and 1.25% skim milk.</description><subject>Amino acids</subject><subject>Bacillus</subject><subject>Bacteria</subject><subject>Clean technology</subject><subject>Food industry</subject><subject>green technology</subject><subject>Leather</subject><subject>Microorganisms</subject><subject>Optimization</subject><subject>Peptides</subject><subject>Protease</subject><subject>Proteinase</subject><subject>Proteolysis</subject><subject>Proteolytic bacteria</subject><subject>Response surface methodology</subject><subject>rRNA 16S</subject><subject>Similarity</subject><subject>Skim milk</subject><subject>Soil bacteria</subject><subject>Soil microorganisms</subject><subject>Strain analysis</subject><subject>Strains (organisms)</subject><issn>1755-1307</issn><issn>1755-1315</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNqFkF1LwzAUhoMoOKe_wYBXXtTmq0nqncj8gIHC3HVI02RmbE1Nuov5622pTATBq3M4ec57wgPAJUY3GEmZY1EUGaa4yDFBOMc56mvJjsDk8HJ86JE4BWcprRHigtFyApavMXRWJwvbGOqd6XxoYLWHKfgNrLTpbPQauhDhKlrbwM6a9yZswmp_CxdmGPlmBXVTw9B2fus_9ZBwDk6c3iR78V2nYPkwe7t_yuYvj8_3d_PMUCJYJqisHZYUE8ecZpJUGhGGKscL7ApRMU4449QiYkosC4ERNYaQ2jpuas0lnYKrMbf__MfOpk6twy42_UlF-liJS05FT4mRMjGkFK1TbfRbHfcKIzU4VIMdNZhSg0OF1eiw37weN31of6Jns8VvTrW161n6B_vfhS8QpoAh</recordid><startdate>20230601</startdate><enddate>20230601</enddate><creator>Ratnaningrum, D</creator><creator>Kosasih, W</creator><creator>Endah, E S</creator><creator>Lathifa, A K N</creator><creator>Diwan, A M</creator><creator>Nida, V</creator><creator>Saraswaty, V</creator><creator>Risdian, C</creator><general>IOP Publishing</general><scope>O3W</scope><scope>TSCCA</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>PATMY</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PYCSY</scope></search><sort><creationdate>20230601</creationdate><title>Protease production by soil bacteria for green technology: Screening and optimization</title><author>Ratnaningrum, D ; Kosasih, W ; Endah, E S ; Lathifa, A K N ; Diwan, A M ; Nida, V ; Saraswaty, V ; Risdian, C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3274-738df18312f4fa482ba0240bf651f57b4626463e02c91857103cc22def6cda683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Amino acids</topic><topic>Bacillus</topic><topic>Bacteria</topic><topic>Clean technology</topic><topic>Food industry</topic><topic>green technology</topic><topic>Leather</topic><topic>Microorganisms</topic><topic>Optimization</topic><topic>Peptides</topic><topic>Protease</topic><topic>Proteinase</topic><topic>Proteolysis</topic><topic>Proteolytic bacteria</topic><topic>Response surface methodology</topic><topic>rRNA 16S</topic><topic>Similarity</topic><topic>Skim milk</topic><topic>Soil bacteria</topic><topic>Soil microorganisms</topic><topic>Strain analysis</topic><topic>Strains (organisms)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ratnaningrum, D</creatorcontrib><creatorcontrib>Kosasih, W</creatorcontrib><creatorcontrib>Endah, E S</creatorcontrib><creatorcontrib>Lathifa, A K N</creatorcontrib><creatorcontrib>Diwan, A M</creatorcontrib><creatorcontrib>Nida, V</creatorcontrib><creatorcontrib>Saraswaty, V</creatorcontrib><creatorcontrib>Risdian, C</creatorcontrib><collection>IOP Journals (Institute Of Physics)</collection><collection>IOPscience (Open Access)</collection><collection>CrossRef</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Agriculture & Environmental Science Database</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>Environmental Science Database</collection><collection>Publicly Available Content (ProQuest)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Environmental Science Collection</collection><jtitle>IOP conference series. Earth and environmental science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ratnaningrum, D</au><au>Kosasih, W</au><au>Endah, E S</au><au>Lathifa, A K N</au><au>Diwan, A M</au><au>Nida, V</au><au>Saraswaty, V</au><au>Risdian, C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protease production by soil bacteria for green technology: Screening and optimization</atitle><jtitle>IOP conference series. Earth and environmental science</jtitle><addtitle>IOP Conf. Ser.: Earth Environ. Sci</addtitle><date>2023-06-01</date><risdate>2023</risdate><volume>1201</volume><issue>1</issue><spage>12094</spage><pages>12094-</pages><issn>1755-1307</issn><eissn>1755-1315</eissn><abstract>Protease is a type of enzyme that hydrolyzes proteins into peptides and amino acids. The demand for protease for green technology in some industries like detergent, food, and leather is increasing nowadays. Some soil bacteria, especially the
Bacillus
strains, are known to have the ability to produce protease. Compared to other sources like plant and animal origin, microbial protease has more advantages as it can be produced at an industrial scale, short cultivation time, and are easy to harvest. This research aimed to screen the proteolytic bacteria from the bulk soil samples collected under mango trees and to study the optimum condition for protease production using the response surface methodology (RSM). Three bacterial strains (SH2CR, SH3CR, and SC4CR) were isolated and shown to have proteolytic activity. Based on 16S rRNA gene analysis, the strain SH2CR was close to
Priestia megaterium
NBRC 15308
T
(96.97% similarity), while the strains SH3CR and SC4CR were related to
Bacillus zanthoxyli
strain 1433
T
(100% similarity). One of them, SH2CR, was further studied using a fermenter at a one-liter production scale with the medium containing skim milk at 30°C. The best condition for protease production by SH2CR was achieved at 48 h incubation time, 300 rpm of agitation, and 1.25% skim milk.</abstract><cop>Bristol</cop><pub>IOP Publishing</pub><doi>10.1088/1755-1315/1201/1/012094</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | IOP conference series. Earth and environmental science, 2023-06, Vol.1201 (1), p.12094 |
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| subjects | Amino acids Bacillus Bacteria Clean technology Food industry green technology Leather Microorganisms Optimization Peptides Protease Proteinase Proteolysis Proteolytic bacteria Response surface methodology rRNA 16S Similarity Skim milk Soil bacteria Soil microorganisms Strain analysis Strains (organisms) |
| title | Protease production by soil bacteria for green technology: Screening and optimization |
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