In vivo Acetylcholinesterase activity and Antioxidant property of Cucurbita pepo ethanolic extract in Alzheimer’s disease induced by Aluminium chloride in Sprague Dawley rat model

• Published in: Research journal of pharmacy and technology 2023-03, Vol.16 (3), p.1065-1071
• Main Authors: Prathap Yadav, Rachagolla Sai, Vijetha Shenoy, Belle, Kumar, Nitesh, Kumar, G Prasanna, Kumar, S Naveen
• Format: Article
• Language: English
• Subjects: Alzheimer's disease; Antioxidants; Brain; Dementia; Disease control; Drug dosages; Enzymes; Fatty acids; Flavonoids; Free radicals; Medicine; Memory; Metabolites; Older people; Oxidative stress; Pathophysiology; Peptides; Phytochemicals; Polyunsaturated fatty acids; Proteins; Seeds; Variance analysis
• ISSN: 0974-3618; 0974-360X; 0974-306X
• DOI: 10.52711/0974-360X.2023.00178

The pathophysiology of Alzheimer's disease (AD) typically begins before symptoms manifest. Early diagnosis and treatment can slow down the disease progression and improve the prognosis. Decreased acetylcholine, antioxidants, and polyunsaturated fatty acids enhance the progression of AD. Phytochemicals present in the Cucurbita pepo (C. pepo) plants may have anti-cholinesterase activity. Therefore, this study was performed using ethanolic extract of pumpkin seeds in the AlCl3 induced AD in Sprague Dawley rats. These rats were categorized into five groups, i.e., control, disease control (AlCl3), standard (Rivastigmine), and two treatment groups, one with 100mg/kg and the other with 200mg/kg of the pumpkin seed extract which was given once daily orally for 28days. The rats were assessed for behavioral and biochemical parameters such as antioxidant enzymes and acetyl-cholinesterase levels in brain homogenate. There was statistically significant (p