Therapeutic effect of Embelin and Levodopa combination in Rotenone induced Parkinson’s disease in mice on Neurobehavioral Changes

• Published in: Research journal of pharmacy and technology 2023-05, Vol.16 (5), p.2107-2114
• Main Authors: Koppal, Anand, H. R, Vagdevi, Sivanesan, Senthilkumar, Ethirajan, Sukumar, Vijayaraghavan, Rajagopalan
• Format: Article
• Language: English
• Subjects: Albinism; Alzheimer's disease; Animal cognition; Blood-brain barrier; Brain research; Dementia; Dopamine; Drug dosages; Laboratories; Medical research; Neurodegeneration; Olive oil; Oxidative stress; Parkinson's disease; R&D; Research & development; Tremor (Muscular contraction)
• ISSN: 0974-3618; 0974-360X; 0974-306X
• DOI: 10.52711/0974-360X.2023.00346

Objective: The present work evaluated the therapeutic potential of embelin monotherapy, embelin plus levodopa (L-Dopa) combination therapy in rotenone induced Parkinson’s disease (PD) model mice. Scope: Based on several growing evidence, antioxidants can be considered as modifiable factors that eventually decrease the risk of degeneration and progression of PD. Especially anti-oxidants from herbal medicines play vital role against several degenerative diseases. Therefore, we evaluated the neuroprotective effect of embelin and embelin plus L-Dopa therapy in rotenone induced behavioural changes in mice. Methods: Swiss Albino male adult mice were randomly divided into 7 groups. Group-1 (Control) 2mL/kg olive oil by oral route (p.o.), Groups from 2 to 7 were induced with 2.5mg/kg rotenone by intraperitoneal route (i.p.), L-Dopa 7.5mg/kg p.o was administered to Group-3. Group-4 and Group-5 were given 20mg/kg and 40mg/kg embelin p.o. Group 6 and 7 were given embelin p.o. at a dose of 20mg and 40mg/kg respectively along with 7.5mg/kg L-Dopa p.o. Result: Administration of rotenone (2.5mg/kg) for twenty-one days to mice consecutively produced motor incoordination, decreased locomotor activity anxiety like behaviours and bradykinesia. Embelin monotherapy as well as combination therapy of embelin along with L-Dopa significantly decreased the effect of rotenone on behavioural changes and rendered neuroprotection in a dose dependant manner.