Biological and molecular characterization of Lasiodiplodia theobromae causing dieback disease of strawberry in Thailand

Strawberry (Fragaria × ananassa) is commercially grown in Chiang Mai Province of Northern Thailand. Strawberry dieback, whose symptoms include dieback of runners and roots followed by wilt and necrotic discoloration in crowns, was first observed in diseased strawberry cultivated in the greenhouse. F...

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Bibliographic Details
Published in:Journal of phytopathology 2023-08, Vol.171 (7-8), p.285-290
Main Authors: Phetphan, Chisanuphong, Supakitthanakorn, Salit, Phuakjaiphaeo, Chatsuda, Pipattanapuckdee, Aimalin, Kunasakdakul, Kaewalin, Ruangwong, On‐Uma
Format: Article
Language:English
Subjects:
Botryosphaeriaceae
Cultivation
Dieback
Discoloration
first report
Fragaria ananassa
Fragaria × ananassa
Fruit cultivation
Fruits
fungal identification
Fungi
Lasiodiplodia theobromae
Microscopes
Pathogenesis
Pathogenicity
Pathogens
plant pathogenic fungi
Polymerase chain reaction
rRNA 28S
Signs and symptoms
Strawberries
Wilt
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Summary:Strawberry (Fragaria × ananassa) is commercially grown in Chiang Mai Province of Northern Thailand. Strawberry dieback, whose symptoms include dieback of runners and roots followed by wilt and necrotic discoloration in crowns, was first observed in diseased strawberry cultivated in the greenhouse. Fungi were isolated from infected parts of diseased strawberry and a pathogenicity assay was conducted for confirmation of fungal pathogenesis. Morphological features of fungal isolates were observed using stereo and compound microscopes. For molecular characterization, internal transcribed spacer (ITS) and large subunit 28S rRNA (LSU) regions were amplified using polymerase chain reaction (PCR) and sequenced. Based on morphological and molecular identification, the fungi were identified as Lasiodiplodia theobromae. Koch's postulates were fulfilled by reisolating the pathogen. To the best of our knowledge, this is the first report of L. theobromae causing dieback of strawberry in Thailand.
ISSN:0931-1785
1439-0434
DOI:10.1111/jph.13181