Structural and Functional Assessment of Human Skin Fibroblasts in the Free State and as Part of a Biomedical Cell Product Using Probe Fluorescence

The key biophysical characteristics of human skin fibroblasts in the free state and as part of a biomedical cell product (cells with Collost gel carrier) were studied using flow cytometry and fluorescent probes. Data were obtained on preserving the intact state of fibroblasts in combination with the...

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Bibliographic Details
Published in:Journal of applied spectroscopy 2023-07, Vol.90 (3), p.515-520
Main Authors: Butenka, A. V., Pinchuk, S. V., Volotovski, I. D.
Format: Article
Language:English
Subjects:
Analytical Chemistry
Atomic/Molecular Structure and Spectra
Calcium ions
Cell membranes
Cytoplasm
Ethylenediaminetetraacetic acid
Fibroblasts
Flow cytometry
Fluorescence
Fluorescent indicators
Investigations
Lipids
Membrane lipids
Physics
Physics and Astronomy
Skin
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Summary:The key biophysical characteristics of human skin fibroblasts in the free state and as part of a biomedical cell product (cells with Collost gel carrier) were studied using flow cytometry and fluorescent probes. Data were obtained on preserving the intact state of fibroblasts in combination with the carrier, namely, maintaining the content of ionized calcium, reactive oxygen species in the cell cytoplasm, and the level of microviscosity of the lipid bilayer of cell membranes. Collost gel was shown in this study not to induce oxidative stress in fibroblasts. Cell membranes were not damaged and there is no increase in the content of calcium ions in the fi broblast cytoplasm. The microviscosity of the lipid bilayer of the membranes does not change, which would suggest the possible feasibility of using Collost gel carrier for the formation of a biomedical cell product in cell therapy.
ISSN:0021-9037
1573-8647
DOI:10.1007/s10812-023-01561-3