A HPLC method for simultaneous quantification of chlorhexidine and thymol using Box-Behnken design for robustness of the method assessment

The antimicrobial chlorhexidine is considered the gold standard in dentistry. Thymol is a phenol found in the essential oils of various plant species and also stands out for its antimicrobial potential. Synergistic effects can be promoted by applying these two active pharmaceutical ingredients toget...

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Bibliographic Details
Published in:Journal of liquid chromatography & related technologies 2023-09, Vol.46 (6-10), p.168-179
Main Authors: Muniz, Vanessa Morais, Chaves Júnior, José Venâncio, Aragão, Cícero Flávio Soares, Souza, Fábio Santos de, Sampaio, Fábio Correia
Format: Article
Language:English
Subjects:
Active control
Antiinfectives and antibacterials
Box-Behnken
Chlorhexidine
Coefficient of variation
Controlled release
Degradation products
Dentistry
Design of experiments
Essential oils
Experimental design
High performance liquid chromatography
HPLC
Ingredients
Ions
Liquid chromatography
Mathematical analysis
Microparticles
Optimization
Pharmaceuticals
Phenols
Plant species
Quality control
Sodium phosphate
Stationary phase
Synergistic effect
Thymol
Triethylamine
validation
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Summary:The antimicrobial chlorhexidine is considered the gold standard in dentistry. Thymol is a phenol found in the essential oils of various plant species and also stands out for its antimicrobial potential. Synergistic effects can be promoted by applying these two active pharmaceutical ingredients together in technological products, for example in controlled release microparticles. The objective of this work was to develop and validate an analytical methodology applying a Box-Behnken experimental design and using High Performance Liquid Chromatography capable of quantifying chlorhexidine and thymol simultaneously in a matrix including pharmaceutical excipients. After optimization, the mobile phase consisted of methanol and 0.03 M monobasic sodium phosphate buffer (60:40), with 0.4% triethylamine and octylsilane as the applied stationary phase. The method proved selective, even in the presence of chlorhexidine and thymol degradation products. For chlorhexidine, the method was linear from 4.8 to 19.2 µg/mL, and for thymol from 8.0 to 32.0 µg/mL. Accuracy was close to 100%, and the precision assessment yielded coefficient variation values of
ISSN:1082-6076
1520-572X
DOI:10.1080/10826076.2023.2227792