Extraction, Purification, and Crystallization of GTPase Era from Staphylococcus aureus

Protein crystal structure studies are an important tool for drug design. The growth of high-quality crystals suitable for X-ray diffraction is the limiting factor and the bottleneck in obtaining the structural data. Here we report the extraction, purification, and crystallization of the protein GTPa...

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Bibliographic Details
Published in:Crystallography reports 2023-04, Vol.68 (2), p.288-292
Main Authors: Klochkova, E. A., Islamov, D. R., Biktimirov, A. D., Rogachev, A. V., Validov, S. Z., Bikmullin, A. G., Simakin, A. V., Peters, G. S., Yusupov, M. M., Usachev, K. S.
Format: Article
Language:English
Subjects:
Bacteria
Crystal growth
Crystal structure
Crystallization
Crystallography and Scattering Methods
Physics
Physics and Astronomy
Proteins
Purification
Single crystals
Structure of Macromolecular Compounds
X-ray diffraction
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Summary:Protein crystal structure studies are an important tool for drug design. The growth of high-quality crystals suitable for X-ray diffraction is the limiting factor and the bottleneck in obtaining the structural data. Here we report the extraction, purification, and crystallization of the protein GTPase Era from the pathogenic bacterium Staphylococcus aureus . In bacterial cells, GTPase Era acts as a ribosome assembly factor. This enzyme is responsible for the cell growth and division. However, its structure is poorly understood. We obtained crystals of Staphylococcus aureus GTPase Era, which can be used in further structural studies by single-crystal X-ray diffraction analysis.
ISSN:1063-7745
1562-689X
DOI:10.1134/S1063774523010133