Functional reconstitution and structural characterization of the plant hormone receptor ETR1 in lipid nanodiscs

The plant hormone receptor ETR1 regulates many highly relevant agronomic processes. Today, significant functional and structural questions remain unanswered regarding its multi-pass transmembrane sensor domain able to bind and respond to the gaseous plant hormone ethylene at femtomolar concentration...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2023-07, Vol.59 (61), p.9344-9347
Main Authors: Lemke, Moritz, Reiners, Jens, Smits, Sander H. J, Lakomek, Nils, Groth, Georg
Format: Article
Language:English
Subjects:
Lipids
Receptors
Structural analysis
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Summary:The plant hormone receptor ETR1 regulates many highly relevant agronomic processes. Today, significant functional and structural questions remain unanswered regarding its multi-pass transmembrane sensor domain able to bind and respond to the gaseous plant hormone ethylene at femtomolar concentrations. A significant reason for this is the lack of structural data on full-length ETR1 in a lipid environment. Herein, we present the functional reconstitution of recombinant full-length ETR1 purified and solubilized from a bacterial host into lipid nanodiscs, allowing the study of the purified plant receptor for the first time in a detergent-free membrane-like environment. The plant hormone receptor ETR1 nanoparticles obtained in this work provide a major methodological breakthrough for further functional and structural studies on a plant key receptor kinase controlling agronomically important traits.
ISSN:1359-7345
1364-548X
DOI:10.1039/d3cc02619a