Knockdown of KLF7 inhibits the differentiation of both intramuscular and subcutaneous preadipocytes in goat

• Published in: Animal biotechnology 2023-11, Vol.34 (4), p.1072-1082
• Main Authors: Li, Yanyan, Xu, Qing, Wang, Yong, Chen, Dingshuang, Du, Yu, Li, Ruiwen, Liu, Kehan, Zhu, Jiangjiang, Lin, Yaqiu
• Format: Article
• Language: English
• Subjects: Adipocytes; Adipogenesis; Binding sites; Biomarkers; Differentiation; goat; Goats; KLF7; Krueppel-like factor; Lipids; Preadipocytes
• ISSN: 1049-5398; 1532-2378
• DOI: 10.1080/10495398.2021.2011739

KLF7 belongs to the Krüppel-like factors (KLFs) family, which function as transcriptional regulators controlling a number of basic cellular processes, involving proliferation, differentiation, and migration. Here, we reveal insights into the differentiated expression of KLF7 in different goat tissues and different stages of growth, and the inhibition role of KLF7 knockdown to differentiation by using goat intramuscular and subcutaneous preadipocytes. We demonstrate that KLF7 expression is obviously changed during the differentiation of preadipocytes into mature adipocytes. Knockdown of KLF7 inhibited lipid droplet accumulation, reduced the expression of adipogenic markers both in intramuscular and subcutaneous preadipocytes in goats, suggesting that KLF7 is a novel regulator of adipogenesis. KLF7 expression changed also up or down-regulation the other KLF family members, but there were differences between these two types of cells. Investigation into the mechanism that KLF7 regulates preadipocyte differentiation revealed that KLF family members KLF1, KLF5, KLF6, KLF8, KLF11, KLF12, KLF16, KLF17 and adipogenic markers C/EBPα and SREBP1 promoter region present KLF7 transcriptional binding sites. Altogether, the data here identify KLF7 as a novel regulator of adipogenesis.