Establishment of liquid phase double antibody radioimmunoassay system for in-vitro determination of erythropoietin hormone in human serum

The aim of study was the production of polyclonal antibody of erythropoietin hormone (Anti-EPO) to determine EPO in human serum by liquid-phase radioimmunoassay (RIA). Production of anti-EPO was performed by immunizing Balb/C mice with EPO antigen using primary immunization and four booster doses. E...

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Bibliographic Details
Published in:Journal of radioanalytical and nuclear chemistry 2023-08, Vol.332 (8), p.3103-3112
Main Authors: Ebeid, N. H., El-Shershaby, Hanan M., Shafik, H. M., Moustafa, K. A.
Format: Article
Language:English
Subjects:
Anemia
Antibodies
Antigens
Chemistry
Chemistry and Materials Science
Diagnostic Radiology
Erythropoietin
Hadrons
Heavy Ions
Hormones
Immunization
Inorganic Chemistry
Liquid phases
Nuclear Chemistry
Nuclear energy
Nuclear Physics
Physical Chemistry
Radioimmunoassay
Tracers (Biology)
Viral antibodies
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Summary:The aim of study was the production of polyclonal antibody of erythropoietin hormone (Anti-EPO) to determine EPO in human serum by liquid-phase radioimmunoassay (RIA). Production of anti-EPO was performed by immunizing Balb/C mice with EPO antigen using primary immunization and four booster doses. EPO tracer ( 125 I-EPO) was prepared using Chloramine-T method. Additionally, EPO standards were mad. Furthermore, the assay was optimized and validated in additional studies. In conclusion, the elaborated EPO-RIA system exhibits a simple, accurate, specific and sensitive method for determining EPO concentration in human serum and might be suitable for clinical diagnosis of myeloproliferative diseases.
ISSN:0236-5731
1588-2780
DOI:10.1007/s10967-023-09011-2