Synergistic in vitro antimicrobial activity of caffeine/AgNPs–triton X-100

• Published in: Applied physics. A, Materials science & processing Materials science & processing, 2023-09, Vol.129 (9), Article 611
• Main Authors: Muthusamy, Prabakaran, Antony, Susaimanikam Arul, Palani, Geetha, Saravanan, D., Chithambaram, V.
• Format: Article
• Language: English
• Subjects: Applied physics; Biosynthesis; Caffeine; Characterization and Evaluation of Materials; Condensed Matter Physics; E coli; Machines; Manufacturing; Materials science; Nanoparticles; Nanotechnology; Optical and Electronic Materials; Physics; Physics and Astronomy; Processes; Silver; Surface plasmon resonance; Surfaces and Interfaces; Thin Films
• ISSN: 0947-8396; 1432-0630
• DOI: 10.1007/s00339-023-06892-8

The aim of this study is to investigate the antimicrobial activity and in vitro toxicity of caffeine/AgNPs–triton X-100 and characterize the produced nanoparticle. In the present investigation, the green biosynthesis of caffeine/AgNPs with triton X-100 has been carried out, and Ultraviolet–Visible Spectroscopy (UV–Vis) analysis was done which revealed an intense surface plasmon resonance (SPR) band at 420 nm, thus confirming the formation of triton X-100. Transmission electron microscopy (TEM) study showed that the nanoparticles were spherical in shape with an average size of 16–24 nm diameter. Using the MTT assay, biosynthesized AgNPs effectively inhibited Hep-G2 cancer cells with a 60.53% reduction.. The antibacterial activity of synthesized AgNPs against a diverse range of Gram-positive pathogens was determined using disk diffusion method and the results showed that caffeine–AgNP–Triton X-100 effectively inhibited E. coli and S. aureus.