Screening of biohydrogen producing endophytic fungi from biodiesel plants

ABSTRACT Biohydrogen from endophytic fungi constitutes an alternative biofuel resource with considerable advantages over biodiesel and ethanol extracted from existing food crops. Endophytes common in plant tissues mimic host physiological behavior and produce primary and secondary metabolites like o...

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Published in:Clean : soil, air, water air, water, 2023-08, Vol.51 (8), p.n/a
Main Authors: Paul, Susmita, Bhagobaty, Ranjan Kumar, Nihalani, Mool Chand, Joshi, Santa Ram
Format: Article
Language:English
Subjects:
Analysis
Bioactive compounds
Biodiesel fuels
Biofuels
Biohydrogen
Chromatography
Deoxyribonucleic acid
Diesel
DNA
Endophytes
Ethanol
Fats
Fungi
Host plants
Lipids
Metabolites
methane
Microorganisms
myco‐diesel
Nucleotide sequence
Oils & fats
Organic compounds
Ozone
Plant tissues
Plants
Secondary metabolites
Statistical analysis
Statistical methods
VOCs
Volatile organic compounds
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Summary:ABSTRACT Biohydrogen from endophytic fungi constitutes an alternative biofuel resource with considerable advantages over biodiesel and ethanol extracted from existing food crops. Endophytes common in plant tissues mimic host physiological behavior and produce primary and secondary metabolites like oils and fats. Therefore, endophytes from 7 selected biodiesel plants, and 30 endophytic fungi isolated from 170 different plant parts were screened. The methodology included staining, lipid analysis, DNA isolation, molecular characterization, identification of fungal isolates by ITS sequences, biodiesel analysis, characterization of volatile organic compounds (VOCs), and statistical analysis. The fungal endophyte isolate SPSRJ28 was the most significant with an accumulation capacity of more than 20% of the total lipid content of its dry biomass, validating its oleaginous nature. In addition, the endophytes investigated in the present study yielded various VOCs that were validated by chromatography for both standards and isolates. When the VOCs were subsequently analyzed for their prospective usage as myco‐diesel, the presence of methane in the isolate SPSRJ28 was found to be highly significant. These findings remarkably support the investigation of endophytic fungi and their relationship with oleaginous plants as alternative sources of bioactive metabolites from microbial cell factories for myco‐diesel generation.
ISSN:1863-0650
1863-0669
DOI:10.1002/clen.202300150