Penicillin Acylase: A Retrospective Study of the Kinetics and Thermodynamics of Practically Significant Reactions

This review considers the contribution of works carried out at the scientific school of Ilya Vasilievich Berezin to research on the kinetics and thermodynamics of penicillin acylase-catalyzed reactions. Methods for determining the activity of penicillin acylases, the reversibility of the enzymatic h...

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Bibliographic Details
Published in:Moscow University chemistry bulletin 2023-08, Vol.78 (4), p.187-200
Main Authors: Panin, N. V., Guranda, D. T., Shapovalova, I. V., Švedas, V. K.
Format: Article
Language:English
Subjects:
Amides
Antibiotics
Cephalosporins
Chemical reactions
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Kinetics
Penicillin
Reagents
Review
Substrates
Thermodynamics
Titration
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Summary:This review considers the contribution of works carried out at the scientific school of Ilya Vasilievich Berezin to research on the kinetics and thermodynamics of penicillin acylase-catalyzed reactions. Methods for determining the activity of penicillin acylases, the reversibility of the enzymatic hydrolysis of a number of penicillins, cephalosporins, and related compounds, the influence of the β-lactam ring on the thermodynamics of the synthesis of new penicillins and cephalosporins by direct condensation as well as by acyl transfer, the issues of optimizing the conditions for enzymatic acyl transfer, and the use of supersaturated reagent solutions are discussed. The role of chromogenic substrates in the study of penicillin acylase, the possibility of using the methods of titration of active sites of the enzyme and the creation of “smart” biocatalysts based on penicillin acylase due to the formation of conjugates with stimulus-sensitive polymers are considered.
ISSN:0027-1314
1935-0260
DOI:10.3103/S0027131423040041