A Novel Multiplex RT-PCR for Simultaneous Detection of Malaria, Chikungunya and Dengue Infection (MCD-RT-PCR)

The “triple whammy” of infection of Malaria, Chikungunya and Dengue can considerably change the spectrum of clinical manifestations that complicates the diagnosis of the disease. No single assay is available for the simultaneous detection of these three pathogens together. We have developed a single...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences, India. Section B: Biological sciences India. Section B: Biological sciences, 2023-09, Vol.93 (3), p.755-761
Main Authors: Sinha, Swati, Gahtori, Renuka, Kumari, Preeti, Baharia, Rajendra K., Saxena, Mritunjay, Pande, Veena, Anvikar, Anupkumar R., Savargaonkar, Deepali
Format: Article
Language:English
Subjects:
Behavioral Sciences
Biomedical and Life Sciences
Chikungunya virus
Dengue fever
Diagnosis
Life Sciences
Malaria
Nucleic Acid Chemistry
Pathogens
Plant Biochemistry
Plasmodium
Polymerase chain reaction
Research Article
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Summary:The “triple whammy” of infection of Malaria, Chikungunya and Dengue can considerably change the spectrum of clinical manifestations that complicates the diagnosis of the disease. No single assay is available for the simultaneous detection of these three pathogens together. We have developed a single-step multiplex RT-PCR assay using in-house designed primers which could detect as low as 0.25 ng/µl of isolated RNA. The assay was evaluated on 104 samples for the detection of these three pathogens. The sensitivity of the assay was found to be 100% for detecting all three pathogens ( P  
ISSN:0369-8211
2250-1746
DOI:10.1007/s40011-023-01447-6