A Novel Multiplex RT-PCR for Simultaneous Detection of Malaria, Chikungunya and Dengue Infection (MCD-RT-PCR)

The “triple whammy” of infection of Malaria, Chikungunya and Dengue can considerably change the spectrum of clinical manifestations that complicates the diagnosis of the disease. No single assay is available for the simultaneous detection of these three pathogens together. We have developed a single...

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Published in:Proceedings of the National Academy of Sciences, India. Section B: Biological sciences India. Section B: Biological sciences, 2023-09, Vol.93 (3), p.755-761
Main Authors: Sinha, Swati, Gahtori, Renuka, Kumari, Preeti, Baharia, Rajendra K., Saxena, Mritunjay, Pande, Veena, Anvikar, Anupkumar R., Savargaonkar, Deepali
Format: Article
Language:English
Subjects:
Behavioral Sciences
Biomedical and Life Sciences
Chikungunya virus
Dengue fever
Diagnosis
Life Sciences
Malaria
Nucleic Acid Chemistry
Pathogens
Plant Biochemistry
Plasmodium
Polymerase chain reaction
Research Article
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container_title Proceedings of the National Academy of Sciences, India. Section B: Biological sciences
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creator Sinha, Swati
Gahtori, Renuka
Kumari, Preeti
Baharia, Rajendra K.
Saxena, Mritunjay
Pande, Veena
Anvikar, Anupkumar R.
Savargaonkar, Deepali
description The “triple whammy” of infection of Malaria, Chikungunya and Dengue can considerably change the spectrum of clinical manifestations that complicates the diagnosis of the disease. No single assay is available for the simultaneous detection of these three pathogens together. We have developed a single-step multiplex RT-PCR assay using in-house designed primers which could detect as low as 0.25 ng/µl of isolated RNA. The assay was evaluated on 104 samples for the detection of these three pathogens. The sensitivity of the assay was found to be 100% for detecting all three pathogens ( P  
doi_str_mv 10.1007/s40011-023-01447-6
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subjects Behavioral Sciences
Biomedical and Life Sciences
Chikungunya virus
Dengue fever
Diagnosis
Life Sciences
Malaria
Nucleic Acid Chemistry
Pathogens
Plant Biochemistry
Plasmodium
Polymerase chain reaction
Research Article
title A Novel Multiplex RT-PCR for Simultaneous Detection of Malaria, Chikungunya and Dengue Infection (MCD-RT-PCR)
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