Cloning and mRNA expression of selenium-dependent glutathione peroxidase from the oyster Magallana hongkongensis in response to cadmium exposure and aerial exposure at different temperatures

• Published in: Fisheries science 2023-09, Vol.89 (5), p.633-646
• Main Authors: Chen, Citing, Gao, Jialong, Zhang, Chaohua, Qin, Xiaoming, Cao, Wenhong, Zheng, Huina, Lin, Haisheng
• Format: Article
• Language: English
• Subjects: 3' Untranslated regions; Air exposure; Biomedical and Life Sciences; Cadmium; Cloning; Complementary DNA; Digestive glands; Exposure; Fish & Wildlife Biology & Management; Food Science; Freshwater & Marine Ecology; Gene expression; Glutathione; Glutathione peroxidase; Life Sciences; Magallana; Mantle; Marine molluscs; Original Article; Oxidative stress; Oysters; Peroxidase; Selenium; Selenocysteine; Sequencing; Specificity; Temperature effects; Tissue
• ISSN: 0919-9268; 1444-2906
• DOI: 10.1007/s12562-023-01704-7

Glutathione peroxidase is an important enzyme in the endogenous antioxidant defense system. In the current study, we cloned the full-length cDNA sequence that encodes a glutathione peroxidase from Magallana hongkongensis (designated as MhGPx ), investigated the tissue expression profile of MhGPx mRNA, and studied the time-dependent effects of cadmium exposure and air exposure at two temperatures (4 °C and 15 °C) on the mRNA level of MhGPx in different tissues (including gill, adductor, mantle, and digestive gland). The full length of MhGPx was 1306 bp. This gene was found to contain a selenocysteine insertion sequence within the 3′ untranslated region, which indicates that the protein it encodes was a selenoprotein. In addition, MhGPx was constitutively expressed in all four selected tissues, and the level of expression in the digestive gland was highest, indicating that MhGPx mRNA expression has tissue-specificity. Under cadmium exposure, mRNA expression was significantly increased on day 1. When exposed to air, the highest MhGPx mRNA expression in the 4 °C group was lower and appeared later than that of the 15 °C group. The results of this study contribute to a better understanding of oyster GPx in response to oxidative stress.