Comparative analysis of single- and dual-wavelength photodynamic therapy regimes with chlorin-based photosensitizers: animal study

Two pronounced absorption peaks in blue and red ranges of the chlorin-based photosensitizer (PS) absorption spectrum provide additional benefits in photodynamic therapy (PDT) performance. Differing optical properties of biological tissues in these ranges allow for both dual-wavelength diagnostics an...

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Bibliographic Details
Published in:Journal of biomedical optics 2020-06, Vol.25 (6), p.063804-063804
Main Authors: Kurakina, Daria, Khilov, Aleksandr, Shakhova, Maria, Orlinskaya, Natalia, Sergeeva, Ekaterina, Meller, Alina, Turchin, Ilya, Kirillin, Mikhail
Format: Article
Language:English
Subjects:
Absorption
Absorption spectra
Biological properties
Comparative analysis
Drug dosages
Edema
Fluorescence
Histopathology
Imaging techniques
Inflammatory diseases
Irradiation
Medical imaging
Monitoring
Optical Coherence Tomography
Optical properties
Photobleaching
Photodynamic therapy
Special Section on Photodynamic Therapy
Tissues
Topical medication
Wavelength
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Summary:Two pronounced absorption peaks in blue and red ranges of the chlorin-based photosensitizer (PS) absorption spectrum provide additional benefits in photodynamic therapy (PDT) performance. Differing optical properties of biological tissues in these ranges allow for both dual-wavelength diagnostics and PDT performance. We provide a comparative analysis of different PDT regimes performed with blue and red lights and their combination, with doses varying from 50 to 150  J  /  cm2. The study was performed on the intact skin of a rabbit ear inner surface, with the use of chlorin e6 as a PS. PDT procedure protocol included monitoring of the treated site with fluorescence imaging technique to evaluate PS accumulation and photobleaching, as well as with optical coherence tomography (OCT) to register morphological and functional responses of the tissue. Optical diagnostic observations were compared with the results of histopathology examination. We demonstrated that PDT procedures with the considered regimes induce weaker organism reaction manifested by edema in normal tissue as compared to irradiation-only exposures with the same light doses. The light doses delivered with red light induce weaker tissue reaction as compared to the same doses delivered with blue light only or with a combination of red and blue lights in equal parts. Results of in-vivo OCT monitoring of tissue reaction are in agreement with the results of histopathology study.
ISSN:1083-3668
1560-2281
DOI:10.1117/1.JBO.25.6.063804