Binding Mechanism of Methylene Blue with Single-Stranded Synthetic Polynucleotides poly(rA) and poly(rU)

The binding of the thiazine dye methylene blue (MB) with single-stranded (ss-) polyribonucleotides poly(rA) and poly(rU) was studied using absorption spectroscopy and UV-melting. The binding mechanisms of MB to poly(rA) and poly(rU) were found to differ significantly. The obtained data indicated tha...

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Bibliographic Details
Published in:Journal of applied spectroscopy 2023-09, Vol.90 (4), p.742-746
Main Authors: Movsesyan, Z. O., Antonyan, A. P., Vardevanyan, P. O.
Format: Article
Language:English
Subjects:
Absorption spectroscopy
Analytical Chemistry
Atomic/Molecular Structure and Spectra
Binding
Methylene blue
Physics
Physics and Astronomy
Polynucleotides
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Summary:The binding of the thiazine dye methylene blue (MB) with single-stranded (ss-) polyribonucleotides poly(rA) and poly(rU) was studied using absorption spectroscopy and UV-melting. The binding mechanisms of MB to poly(rA) and poly(rU) were found to differ significantly. The obtained data indicated that the main mode of interaction between MB and ss-poly(rA) and ss-poly(rU) corresponded to semi-intercalation to electrostatic binding, respectively. MB was also found to contribute to the formation of the hybrid duplex poly(rA)–poly(rU).
ISSN:0021-9037
1573-8647
DOI:10.1007/s10812-023-01590-y