Evaluation of Toxicity and Genotoxicity of Concrete Cast with Steel Slags Using Higher Terrestrial Plants

• Published in: Environmental toxicology and chemistry 2023-10, Vol.42 (10), p.2193-2200
• Main Authors: Alias, Carlotta, Piovani, Giovanna, Benassi, Laura, Abbà, Alessandro, Sorlini, Sabrina, Gelatti, Umberto, Zerbini, Ilaria, Feretti, Donatella
• Format: Article
• Language: English
• Subjects: Aberration; Allium cepa; Bioassays; Bulbs; Chromosome aberrations; Chromosomes; Concrete mixes; Cucumis sativus; Damage assessment; Deoxyribonucleic acid; DNA; DNA damage; Genotoxicity; Leachates; Leaching; Lepidium sativum; Mixtures; Phytotoxicity; Plant cells; Recycled materials; Seedlings; Seeds; Slag; Steel; Toxicology
• ISSN: 0730-7268; 1552-8618
• DOI: 10.1002/etc.5709

The potential impact of concrete mixtures containing steel slag (SS) as a partial replacement of natural aggregates (NA) on the terrestrial ecosystem was assessed using a battery of plant-based bioassays. Leaching tests were conducted on four concrete mixtures and one mixture containing only NA (reference concrete). Leachates were tested for phytotoxicity using seeds of Lepidium sativum, Cucumis sativus, and Allium cepa. Emerging seedlings of L. sativum and A. cepa were used to assess DNA damage (comet test). The genotoxicity of the leachates was also analyzed with bulbs of A. cepa using the comet and chromosome aberration tests. None of the samples caused phytotoxic effects. On the contrary, almost all the samples supported the seedlings; and two leachates, one from the SS-containing concrete and the other from the reference concrete, promoted the growth of C. sativus and A. cepa. The DNA damage of L. sativum and A. cepa seedlings was significantly increased only by the reference concrete sample. In contrast, the DNA damage in A. cepa bulbs was significantly enhanced by the reference concrete but also by that of a concrete sample with SS. Furthermore, all leachates caused an increase in chromosomal aberrations in A. cepa bulbs. Despite some genotoxic effects of the concrete on plant cells, the partial replacement of SS does not seem to make the concrete more hazardous than the reference concrete, suggesting the potential use of SS as a reliable recycled material. Environ Toxicol Chem 2023;00:1-8. © 2023 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.