Role of PGPR and silver nanoparticles on the physiology of Momordica charantia L. irrigated with polluted water comprising high Fe and Mn

• Published in: International journal of phytoremediation 2023-10, Vol.25 (12), p.1643-1655
• Main Authors: Tariq, Shiza, Bano, Asghari
• Format: Article
• Language: English
• Subjects: Ag-nanoparticles; Bioremediation; Carotenoids; Cell culture; Drinking water; Ethanol; Flavonoids; Germination; Iron; Irrigation water; Manganese; Mn and Fe co-contamination; Momordica charantia; Nanoparticles; Organic matter; Oxidative stress; Plant bacterial diseases; Plant biomass; Plant growth; Planting; Proline; Pseudomonas; Pseudomonas stutzeri; Residual effects; Rhizosphere; Seeds; Silver; Wastewater; Water pollution; Well water
• ISSN: 1522-6514; 1549-7879
• DOI: 10.1080/15226514.2023.2180288

The current investigation designed to estimate the bioremediation potential of plant growth-promoting rhizobacteria (PGPR) and Ag-nanoparticles. Tube well and HIT water comprising Mn and Fe above recommended values were used as treatments while tap water irrigation was treated as control. The HIT water showed 24, 200, and 64.11% higher content of Na, K Ca over control. Seeds were sterilized in 95% ethanol and soaked for 3 h before sowing in 73 h old culture of Pseudomonas stutzeri (Kx574858) @ 10 8  cells/ml. Phytotoxic effect of Fe and Mn reduce plant biomass and suppress photosynthetic activity indicates. The carotenoids, proline, and proline activity were 366, 450, and 678% higher in tube well water with combined PGPR and Ag-nanoparticles treatments. Pseudomonas stutzeri was more effective than Ag-nanoparticles to reduce oxidative stress with higher production of carotenoids, flavonoids, proline content, and enzyme SOD and CAT activities in HIT water. It is contingent that the high Mn and Fe bearing waste water enhance PGPR bioremediation potential to reduce metal stress in plants with synergistic action of PGPR and organic matter to alleviate oxidative stresses under metal stress. The residual effect of P. stutzeri on organic matter content of the rhizosphere soil and germination rate was higher for Momordica charantia L. This is the first statement indicating that Ag-nanoparticles oxidize Mn and Fe efficiently to reduce COD and organic matter works synergistically with PGPR and Ag-nanoparticles to enhance ROS production that increase proline, carotenoids, flavonoids, phenolics, and enzymes SOD, POD, PAL, and CAT activities to reduce oxidative stress in cucurbits.