Phage Antibodies for Kanamycin Detection

Recombinant antibodies specific to kanamycin were obtained using a sheep display library of scFv fragments (Griffin.1) and the possibility of their use for the determination of kanamycin by dot-immunoassay was demonstrated. The minimum detectable concentration of kanamycin was 1 μg/mL (distinguishab...

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Bibliographic Details
Published in:Applied biochemistry and microbiology 2023-10, Vol.59 (5), p.716-722
Main Authors: Guliy, O. I., Evstigneeva, S. S., Staroverov, S. A., Fomin, A. S., Karavaeva, O. A.
Format: Article
Language:English
Subjects:
Ampicillin
Antibiotics
Biochemistry
Biomedical and Life Sciences
Gentamicin
Immunoassay
Kanamycin
Life Sciences
Medical Microbiology
Microbiology
Monoclonal antibodies
Neomycin
Phages
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Summary:Recombinant antibodies specific to kanamycin were obtained using a sheep display library of scFv fragments (Griffin.1) and the possibility of their use for the determination of kanamycin by dot-immunoassay was demonstrated. The minimum detectable concentration of kanamycin was 1 μg/mL (distinguishable label binding other than background). It has been shown that anti-kanamycin phage antibodies are specific for kanamycin and do not interact with other antibiotics (neomycin, tetracycline, ampicillin, and gentamicin). Anti-kanamycin phage antibodies are a promising alternative to monoclonal antibodies for use in the determination of kanamycin.
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683823050071