Bioinformatics and gene expression analysis of the legume F-box/WD40 proteins in NaCl and high temperature stress

In this study we aimed to analyze legume F-box/WD40 protein sequences bioinformatically and to test their gene expression profiles during NaCl and high temperature stress. We examined the molecular responses to these stressors for genes encoding subunits of the Skp, Cullin, F-box (SCF) complex in be...

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Bibliographic Details
Published in:Genetic resources and crop evolution 2023-12, Vol.70 (8), p.2637-2655
Main Authors: Çelik, Haluk, Aravena, Andres, Turgut Kara, Neslihan
Format: Article
Language:English
Subjects:
Abiotic stress
Agriculture
Beans
Bioinformatics
Biomedical and Life Sciences
Cdc34 protein
Chickpeas
Cullin
Gene expression
Genomes
Heat resistance
High temperature
Legumes
Life Sciences
Plant Genetics and Genomics
Plant Physiology
Plant Sciences
Plant Systematics/Taxonomy/Biogeography
Proteins
Research Article
Sodium chloride
Stresses
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Summary:In this study we aimed to analyze legume F-box/WD40 protein sequences bioinformatically and to test their gene expression profiles during NaCl and high temperature stress. We examined the molecular responses to these stressors for genes encoding subunits of the Skp, Cullin, F-box (SCF) complex in beans, chickpeas, and barrel medic, including the F-box domain and WD40 repeats. High temperature and salinity stresses were applied to in vitro grown legumes. Two conserved F-box/WD40 proteins were identified in each genome and named MET30 and WD40/YVTN. We examined their gene expression profiles under NaCl and high temperature stress to determine their response and potential functions in response to abiotic stresses. A computational prediction of their 3-dimensional structures suggests that they are conserved among legumes. Our results show that the legume MET30 and WD40/YVTN proteins may have direct roles in the response to high temperature and NaCl stresses. We conclude that the legume proteins MET30 and WD40/YVTN may have a role in the Cdc34/SCF complex based on sequence similarities and expression profiles. Also, according to their response to abiotic stresses, our results suggest that these proteins play an active role in responding to NaCl and high temperature.
ISSN:0925-9864
1573-5109
DOI:10.1007/s10722-023-01592-x