Determination of the Stability Constants of Supramolecular Complexes of Ester Derivatives of Betulin with Randomly Methylated β-Cyclodextrin by Affinity Capillary Electrophoresis

• Published in: Russian Journal of Physical Chemistry A 2023-10, Vol.97 (10), p.2225-2230
• Main Authors: Sursyakova, V. V., Levdansky, V. A., Rubaylo, A. I.
• Format: Article
• Language: English
• Subjects: Affinity; Chemistry; Chemistry and Materials Science; Cyclodextrins; Electrophoresis; Inclusion complexes; Logarithms; Nanomaterials; Physical Chemistry; Physical Chemistry of Nanoclusters; Sodium tetraborate; Stability; Stability constants; Supramolecular Structures
• ISSN: 0036-0244; 1531-863X
• DOI: 10.1134/S0036024423100230

Using affinity capillary electrophoresis, the stability constants of inclusion complexes of a number of betulin ester derivatives with randomly methylated β-cyclodextrin in 0.0100 M sodium tetraborate (pH 9.18, ionic strength 0.0200 M) at 25°С were determined for the first time. The decimal logarithms of the stability constants of 1 : 1 complexes of betulin 3,28-diphthalate, betulin 3,28-disulfate, and betulin 3,28-disuccinate in a 95% confidence interval were 4.64 ± 0.05, 4.78 ± 0.02, and 4.94 ± 0.02, respectively. The logarithms of the stability constants of 1 : 2 complexes of betulin 3,28-diphthalate and betulin 3,28-disulfate were 7.91 (6.66–8.19) and 8.02 (7.63–8.23), respectively. It was determined that betulin 3,28-disuccinate forms only 1 : 1 complex. The obtained stability constants of 1 : 1 complexes are generally higher than those of the complexes of the same compounds with β-cyclodextrin (β-CD) and lower than those for dimethyl-β-CD, γ-CD, and (2-hydroxypropyl)-γ-CD complexes.