Molecular characterization and phylogenetic analysis of Marek's disease virus in chickens from Ogun State, Nigeria

• Published in: Avian pathology 2023-11, Vol.52 (6), p.401-411
• Main Authors: Oluwayinka, E. B., Otesile, E. B., Oni, O. O., Ajayi, O. L., Dunn, J. R.
• Format: Article
• Language: English
• Subjects: Amino acids; Analysis; Arginine; Chickens; Commercial farms; Farms; Genes; In vivo methods and tests; Lesions; Lymphocytes; Marek's disease; Marek's disease virus; meq gene; Microbiological strains; Molecular characterization; Morbidity; Nigeria; Nucleotide sequence; PCR; phylogenetic analysis; Phylogenetics; Phylogeny; Polymerase chain reaction; Poultry; Poultry farming; pp38 gene; Pp38 protein; Proline; Proteins; Strains (organisms); vIL-8 gene; Virulence; Viruses
• ISSN: 0307-9457; 1465-3338
• DOI: 10.1080/03079457.2023.2243838

Marek's disease (MD) is caused by oncogenic MD virus serotype 1 (MDV1) and is characterized by lymphoproliferative lesions resulting in high morbidity and mortality in chickens. Despite being ubiquitous on poultry farms, there is a dearth of information on its molecular characteristics in Nigeria. This study aimed at characterizing three virulence genes (Meq, pp38, and vIL-8) of MDV1 from chickens in Ogun state, Nigeria. Blood, feather quill, and tumour samples of chickens from different commercial poultry farms in Ogun State were pooled, spotted on 107 FTA cards, and screened for MDV1 by polymerase chain reaction (PCR). Phylogenetic analysis was carried out to compare Nigerian MDV1 Meq, pp38, and vIL-8 genes sequences with the published references. Thirteen samples were MDV1-positive and the Meq, as well as pp38, and vIL-8 genes from the different samples were 100% identical. The Meq genes contained 339 amino acids (aa) with three PPPP motifs in the transactivation domain and two interruptions of the PPPP motifs due to proline-to-arginine substitutions at positions 176 and 217 resulting in a 20.88% proline composition. Phylogenetic analysis revealed that the Meq gene clustered with strains from Egypt and very virulent ATE2539 strain from Hungary. Mutations were observed in the pp38 protein (at positions 107 and 109) and vIL-8 protein (at positions 4 and 31). Based on the molecular analysis of the three genes, the results indicate the presence of MDV1 with virulence signatures; therefore, further studies on in vivo pathotyping of Nigerian MDV1 from all states should be performed. RESEARCH HIGHLIGHTS Meq, pp38 and vIL-8 genes were 100% identical between Nigerian MDV strains. Proline content in Nigerian meq gene was 20.88% with two PPPP motifs interruptions. Meq, pp38 and vIL-8 genes of Nigerian MDV were similar to Egyptian and Indian strains.