Molecular engineering of nanobodies as tools in allergology: diagnostics and beyond

Summary Background Molecular technologies have paved the way to improved understanding of allergic diseases in many ways, ranging from molecular allergens to tailor-made tools for analytical, diagnostic, and therapeutic purposes. Engineering of such molecules has become a mainstay in most biotechnic...

Full description

Saved in:
Bibliographic Details
Published in:Allergo journal international 2023, Vol.32 (7), p.240-250
Main Authors: Baunvig Aagaard, Josephine, Ravn Ballegaard, Anne-Sofie, Ommen Andersen, Pernille, Spillner, Edzard
Format: Article
Language:English
Subjects:
Allergens
Allergies
Allergology
Antibodies
Dermatology
Engineering
Environmental Health
Immunology
Medicine
Medicine & Public Health
Review
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Summary Background Molecular technologies have paved the way to improved understanding of allergic diseases in many ways, ranging from molecular allergens to tailor-made tools for analytical, diagnostic, and therapeutic purposes. Engineering of such molecules has become a mainstay in most biotechnical and biomedical areas. A not so new kid on the block is the nanobody, a single-domain antibody obtained from primarily camelid species. Despite their large promise and potential, it took nanobodies a long time to also enter the stage in allergology. Methods This review summarizes the state of the art and the feasibility of engineering nanobody-based tools for applications in allergology. Results In recent years, nanobodies with specificity for allergens have been increasingly generated. In parallel, their molecular engineering has enabled the development of derivatives that offer many advantages compared to standard antibody approaches. Hence, different application forms of nanobody-based molecules have been developed and reported in proof-of-concept studies. Discussion Recent studies give a first glimpse of the future possibilities of nanobody technologies in a complex system such as allergic diseases. It has become clear that the simplicity of the approaches as compared to regular antibody technologies will both broaden and deepen the scope of applications in allergology.
ISSN:2197-0378
2197-0378
DOI:10.1007/s40629-023-00261-w