Inoculation with Biofilm of Bacillus subtilis Promotes the Growth of Lactuca sativa

In Argentina, the excessive use of fertilizers is common in intensively cultivated zones around highly populated areas. Bioinoculants based on plant growth promoting rhizobacteria (PGPR) could be effective for crop production improvement without negative effects on the environment. The objective of...

Full description

Saved in:
Bibliographic Details
Published in:Sustainability 2023-11, Vol.15 (21), p.15406
Main Authors: Sarti, Gabriela Cristina, Galelli, Mirta Esther, Arreghini, Silvana, Cristóbal-Miguez, Josefina Ana Eva, Curá, José Alfredo, Paz-González, Antonio
Format: Article
Language:English
Subjects:
Antibiotics
Bacteria
Biofilms
Chromatography
Emissions
Food supply
Fungi
Glycerol
Horticulture
Microorganisms
Plant growth
Sustainability
Sustainable agriculture
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In Argentina, the excessive use of fertilizers is common in intensively cultivated zones around highly populated areas. Bioinoculants based on plant growth promoting rhizobacteria (PGPR) could be effective for crop production improvement without negative effects on the environment. The objective of this work was to evaluate an alternative inoculation method, namely the application of the biofilm produced by Bacillus subtilis as a growth promoter on seeds of three varieties of Lactuca sativa, and to compare it with the common planktonic approach. Biofilm was obtained under static culture conditions, while planktonic inoculum was produced at 150 rpm. The major biofilm effects were observed with Bacillus subtilis subsp. spizizenii, that showed antifungal activity against phytopathogens, synthesized plant growth regulators (IAA, cytokinin and ABA) and solubilized phosphates. The Grand Rapid variety inoculated with biofilm showed the best results, with 30% and 37% higher aerial and root biomass, respectively, compared to the planktonic form. Moreover, the biofilm positive effects were observed through successive plant development stages until harvest, when the bacterium was recovered from the interior of the roots. The biofilm of B. subtilis subsp. spizizenii behave as a superior growth-promoting inoculant compared to the traditional planktonic inoculation technique.
ISSN:2071-1050
2071-1050
DOI:10.3390/su152115406