Alpha-amylase-assisted extraction of protein concentrates from Raphanus sativus L. leaves

This research focused on the preparation of radish leaf protein concentrates (RLPC) by applying enzyme-assisted extraction, where α-amylase, protease, and xylanase were employed for the same. The α-amylase-assisted extraction showed maximum extraction yield (10.22%) and protein content (66.93%). The...

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Bibliographic Details
Published in:Biomass conversion and biorefinery 2023-11, Vol.13 (16), p.15051-15065
Main Authors: Kaur, Gurkanwal, Bhatia, Surekha
Format: Article
Language:English
Subjects:
Amino acids
Amylases
Biotechnology
Energy
Fractionation
Globulins
Methionine
Microorganisms
Original Article
Proteins
Radishes
Renewable and Green Energy
Response surface methodology
Tryptophan
Xylanase
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Summary:This research focused on the preparation of radish leaf protein concentrates (RLPC) by applying enzyme-assisted extraction, where α-amylase, protease, and xylanase were employed for the same. The α-amylase-assisted extraction showed maximum extraction yield (10.22%) and protein content (66.93%). Therefore, this method was optimized using response surface methodology where optimized conditions of extraction temperature (42.8 °C), amylase concentration (18,446 U), and extraction time (4.44 h) resulted in an extraction yield of 9.56% and protein content of 89.41% in the extracted RLPC. Fractionation of the protein concentrate demonstrated the maximum presence of glutelins followed by prolamins, albumins, and globulins. The apparent molecular weights of the RLPC and its isolated fractions ranged between 35 and 92 kDa. The RLPC showed a high in vitro protein digestibility (92.17%), considerable antioxidant activity (DPPH, FRAP, ABTS), and desirable structural and functional properties (water and oil holding capacity, emulsion capacity and stability, least gelation concentration, etc.). Threonine, methionine, and tryptophan were found to be the most abundant amino acids present in the RLPC. The microbial load of the stored RLPC was observed to be in acceptable range during 6 weeks of storage under ambient and refrigerated temperature conditions. Conclusively, α-amylase-extracted RLPC serves as a potential alternative edible plant-based protein fortification source for various food formulations. Graphical Abstract
ISSN:2190-6815
2190-6823
DOI:10.1007/s13399-022-03611-w