Effect of the addition of fungal extracellular biogenic zinc oxide nanoparticles on the in vitro multiplication of Agave salmiana shoots

Using nanoparticles in agriculture requires a positive and eco-friendly response and a better understanding of their effects on plant cultures. Micropropagation is a recourse for the mass production and rescue of plant species that uses biotechnological tools in which it is possible to include nanop...

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Published in:Plant cell, tissue and organ culture tissue and organ culture, 2023-11, Vol.155 (2), p.479-491
Main Authors: Canales-Mendoza, Ana Itsel, Villanueva-Ibáñez, Maricela, Tovar-Jiménez, Xochilt, Álvarez-Cervantes, Jorge
Format: Article
Language:English
Subjects:
Agave
Biomedical and Life Sciences
Biotechnology
Cell culture
Filtrate
Growth regulators
Life Sciences
Mass production
Microbial contamination
Microorganisms
Micropropagation
Multiplication
Nanoparticles
Nanotechnology and Nanoparticles in Vitro
Organogenesis
Original Article
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Plant Sciences
Plant species
Seedlings
Shoots
Stomata
Zinc oxide
Zinc oxides
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Summary:Using nanoparticles in agriculture requires a positive and eco-friendly response and a better understanding of their effects on plant cultures. Micropropagation is a recourse for the mass production and rescue of plant species that uses biotechnological tools in which it is possible to include nanoparticles for different purposes. In this study, zinc oxide nanoparticles (ZNPs) with an average size of 70 nm were biosynthesized with cell-free filtrate (CFF) from Mucor fragilis , the addition of ZNPs to the shoots of Agave salmiana var. Ayoteco, in their multiplication stage, was evaluated. Agave seedlings treated with ZNPs generally showed organogenesis after 20 days, with an optimal production of 18 shoots. Depending on the treatment applied, the combination of NPs and intermediate concentrations of the studied regulators caused a modification of the structures in the shoots and seedlings, mainly in the stomata, without the accumulation of ZnO. The antioxidant activity in shoots and seedlings depended on the stress generated by abiotic agents, such as growth regulators and NPs, to which they were exposed. Key message The combination of extracellular biogenic zinc oxide nanoparticles and growth regulators reduced the time required for shoot generation in A. salmiana while simultaneously preventing microbial contamination of the culture medium.
ISSN:0167-6857
1573-5044
DOI:10.1007/s11240-023-02589-1